• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

沉默的长链非编码RNA DDX11-AS1或上调的微小RNA-34a-3p通过抑制TRAF5来抑制肝癌细胞的恶性表型。

Silenced lncRNA DDX11-AS1 or up-regulated microRNA-34a-3p inhibits malignant phenotypes of hepatocellular carcinoma cells via suppression of TRAF5.

作者信息

Ding Gangqiang, Zeng Yanli, Yang Dongqiang, Zhang Can, Mao Chongshan, Xiao Erhui, Kang Yi, Shang Jia

机构信息

Department of Infectious Diseases, Henan Key Laboratory for Liver Disease, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, No. 7 Weiwu Road, Zhengzhou, 450003, Henan, China.

出版信息

Cancer Cell Int. 2021 Mar 22;21(1):179. doi: 10.1186/s12935-021-01847-6.

DOI:10.1186/s12935-021-01847-6
PMID:33752668
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7983398/
Abstract

BACKGROUND

Studies have discussed long noncoding RNA DDX11-AS1 (DDX11-AS1)-mediated downstream mechanism in hepatocellular carcinoma (HCC). The goal of this study was to investigate the regulatory mechanism of DDX11-AS1-mediated microRNA-34a-3p (miR-34a-3p)/tumor necrosis factor receptor-associated factor 5 (TRAF5) axis on HCC cells.

METHODS

DDX11-AS1, miR-34a-3p and TRAF5 expression levels in HCC were detected. The correlation of DDX11-AS1, miR-34a-3p and TRAF5 in HCC patients was analyzed by Pearson test. HCC cells were transfected with corresponding plasmid/oligonucleotide, and cell proliferation, migration, invasion, apoptosis and tumor formation ability were detected. Bioinformatics software, dual luciferase report experiment and RNA-pull down experiment analysis were applied to verify the targeting relationship between DDX11-AS1, miR-34a-3p and TRAF5.

RESULTS

Elevated DDX11-AS1 and TRAF5 and reduced miR-34a-3p exhibited in HCC. Silenced DDX11-AS1 or up-regulated miR-34a-3p inhibited the proliferation, migration, invasion, promoted apoptosis of HCC cells and repressed the tumor growth in nude mice. In addition, DDX11-AS1 bound to miR-34a-3p to target TRAF5. Silencing TRAF5 or elevating miR-34a-3p expression mitigated up-regulated DDX11-AS1-mediated promotion of tumor growth.

CONCLUSION

Silenced DDX11-AS1 or up-regulated miR-34a-3p inhibits HCC cell growth via elevation of TRAF5, which could be of great benefit to find early diagnostic markers for HCC patients.

摘要

背景

已有研究探讨了长链非编码RNA DDX11-AS1（DDX11-AS1）在肝细胞癌（HCC）中的下游调控机制。本研究旨在探究DDX11-AS1介导的微小RNA-34a-3p（miR-34a-3p）/肿瘤坏死因子受体相关因子5（TRAF5）轴对HCC细胞的调控机制。

方法

检测HCC中DDX11-AS1、miR-34a-3p和TRAF5的表达水平。采用Pearson检验分析HCC患者中DDX11-AS1、miR-34a-3p和TRAF5的相关性。用相应的质粒/寡核苷酸转染HCC细胞，检测细胞的增殖、迁移、侵袭、凋亡及肿瘤形成能力。应用生物信息学软件、双荧光素酶报告实验和RNA下拉实验分析来验证DDX11-AS1、miR-34a-3p和TRAF5之间的靶向关系。

结果

HCC中DDX11-AS1和TRAF5表达升高，miR-34a-3p表达降低。沉默DDX11-AS1或上调miR-34a-3p可抑制HCC细胞的增殖、迁移、侵袭，促进细胞凋亡，并抑制裸鼠肿瘤生长。此外，DDX11-AS1与miR-34a-3p结合靶向TRAF5。沉默TRAF5或提高miR-34a-3p表达可减轻DDX11-AS1上调介导的肿瘤生长促进作用。

结论

沉默DDX11-AS1或上调miR-34a-3p可通过提高TRAF5水平抑制HCC细胞生长，这可能有助于寻找HCC患者的早期诊断标志物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f37/7983398/c92e78459a3e/12935_2021_1847_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f37/7983398/3c203ccac70c/12935_2021_1847_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f37/7983398/c5421d30279b/12935_2021_1847_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f37/7983398/caab0f0c2740/12935_2021_1847_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f37/7983398/a1073c6b15e1/12935_2021_1847_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f37/7983398/700a45cbd8e6/12935_2021_1847_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f37/7983398/56a7868e6495/12935_2021_1847_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f37/7983398/f8d4d431f297/12935_2021_1847_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f37/7983398/b3921ea50d20/12935_2021_1847_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f37/7983398/b4da4fb72b3a/12935_2021_1847_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f37/7983398/c92e78459a3e/12935_2021_1847_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f37/7983398/3c203ccac70c/12935_2021_1847_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f37/7983398/c5421d30279b/12935_2021_1847_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f37/7983398/caab0f0c2740/12935_2021_1847_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f37/7983398/a1073c6b15e1/12935_2021_1847_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f37/7983398/700a45cbd8e6/12935_2021_1847_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f37/7983398/56a7868e6495/12935_2021_1847_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f37/7983398/f8d4d431f297/12935_2021_1847_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f37/7983398/b3921ea50d20/12935_2021_1847_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f37/7983398/b4da4fb72b3a/12935_2021_1847_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f37/7983398/c92e78459a3e/12935_2021_1847_Fig10_HTML.jpg

相似文献

1
Silenced lncRNA DDX11-AS1 or up-regulated microRNA-34a-3p inhibits malignant phenotypes of hepatocellular carcinoma cells via suppression of TRAF5.沉默的长链非编码RNA DDX11-AS1或上调的微小RNA-34a-3p通过抑制TRAF5来抑制肝癌细胞的恶性表型。
Cancer Cell Int. 2021 Mar 22;21(1):179. doi: 10.1186/s12935-021-01847-6.
2
LncRNA DDX11-AS1 accelerates hepatocellular carcinoma progression via the miR-195-5p/MACC1 pathway.长链非编码RNA DDX11-AS1通过miR-195-5p/MACC1途径加速肝癌进展。
Ann Hepatol. 2021 Jan-Feb;20:100258. doi: 10.1016/j.aohep.2020.09.003. Epub 2020 Sep 19.
3
Correction to: Silenced lncRNA DDX11-AS1 or up-regulated microRNA-34a-3p inhibits malignant phenotypes of hepatocellular carcinoma cells via suppression of TRAF5.更正：沉默的长链非编码RNA DDX11-AS1或上调的微小RNA-34a-3p通过抑制TRAF5抑制肝癌细胞的恶性表型。
Cancer Cell Int. 2021 Dec 9;21(1):659. doi: 10.1186/s12935-021-02360-6.
4
The long noncoding RNA OTUD6B-AS1 enhances cell proliferation and the invasion of hepatocellular carcinoma cells through modulating GSKIP/Wnt/β-catenin signalling via the sequestration of miR-664b-3p.长链非编码 RNA OTUD6B-AS1 通过海绵吸附 miR-664b-3p 调控 GSKIP/Wnt/β-catenin 信号通路增强肝癌细胞的增殖和侵袭。
Exp Cell Res. 2020 Oct 1;395(1):112180. doi: 10.1016/j.yexcr.2020.112180. Epub 2020 Jul 15.
5
C1QTNF1-AS1 regulates the occurrence and development of hepatocellular carcinoma by regulating miR-221-3p/SOCS3.C1QTNF1-AS1 通过调节 miR-221-3p/SOCS3 调控肝细胞癌的发生发展。
Hepatol Int. 2019 May;13(3):277-292. doi: 10.1007/s12072-019-09944-5. Epub 2019 May 8.
6
Long non-coding RNA DDX11-AS1 promotes esophageal carcinoma cell proliferation and migration through regulating the miR-514b-3p/RBX1 axis.长链非编码 RNA DDX11-AS1 通过调控 miR-514b-3p/RBX1 轴促进食管癌细胞的增殖和迁移。
Bioengineered. 2021 Dec;12(1):3772-3786. doi: 10.1080/21655979.2021.1940617.
7
LncRNA SATB2-AS1 overexpression represses the development of hepatocellular carcinoma through regulating the miR-3678-3p/GRIM-19 axis.长链非编码RNA SATB2-AS1的过表达通过调控miR-3678-3p/GRIM-19轴抑制肝细胞癌的发展。
Cancer Cell Int. 2023 Apr 28;23(1):82. doi: 10.1186/s12935-023-02901-1.
8
LncRNA TMPO-AS1 promotes hepatocellular carcinoma cell proliferation, migration and invasion through sponging miR-329-3p to stimulate FOXK1-mediated AKT/mTOR signaling pathway.长链非编码 RNA TMPO-AS1 通过海绵吸附 miR-329-3p 来刺激 FOXK1 介导的 AKT/mTOR 信号通路，从而促进肝癌细胞的增殖、迁移和侵袭。
Cancer Med. 2020 Jul;9(14):5235-5246. doi: 10.1002/cam4.3046. Epub 2020 May 27.
9
LncRNA CDKN2B-AS1 contributes to tumorigenesis and chemoresistance in pediatric T-cell acute lymphoblastic leukemia through miR-335-3p/TRAF5 axis.长链非编码RNA CDKN2B-AS1通过miR-335-3p/TRAF5轴促进小儿T细胞急性淋巴细胞白血病的肿瘤发生和化疗耐药。
Anticancer Drugs. 2020 Sep 25. doi: 10.1097/CAD.0000000000001001.
10
The long non-coding RNA DDX11-AS1 facilitates cell progression and oxaliplatin resistance via regulating miR-326/IRS1 axis in gastric cancer.长链非编码 RNA DDX11-AS1 通过调控 miR-326/IRS1 轴促进胃癌细胞的进展和奥沙利铂耐药性。
Eur Rev Med Pharmacol Sci. 2020 Mar;24(6):3049-3061. doi: 10.26355/eurrev_202003_20669.

引用本文的文献

1
Long noncoding RNA DDX11-AS1 represses sorafenib-induced ferroptosis in hepatocellular carcinoma cells via Nrf2-Keap1 pathway.长链非编码RNA DDX11-AS1通过Nrf2-Keap1途径抑制索拉非尼诱导的肝癌细胞铁死亡。
Discov Oncol. 2024 Oct 10;15(1):544. doi: 10.1007/s12672-024-01431-0.
2
Tumor necrosis factor receptor-associated factor 5 protects against intimal hyperplasia by regulation of macrophage polarization via directly targeting PPARγ.肿瘤坏死因子受体相关因子 5 通过直接靶向 PPARγ 调节巨噬细胞极化来防止内膜增生。
Inflamm Res. 2024 Jun;73(6):929-943. doi: 10.1007/s00011-024-01875-8. Epub 2024 Apr 20.
3
Identifying the programmed cell death index of hepatocellular carcinoma for prognosis and therapy response improvement by machine learning: a bioinformatics analysis and experimental validation.

本文引用的文献

1
The resistance of esophageal cancer cells to paclitaxel can be reduced by the knockdown of long noncoding RNA DDX11-AS1 through TAF1/TOP2A inhibition.通过TAF1/TOP2A抑制作用敲低长链非编码RNA DDX11-AS1可降低食管癌细胞对紫杉醇的耐药性。
Am J Cancer Res. 2019 Oct 1;9(10):2233-2248. eCollection 2019.
2
Overexpression of miRNA-410-3p protects hypoxia-induced cardiomyocyte injury via targeting TRAF5.miRNA-410-3p 的过表达通过靶向 TRAF5 保护低氧诱导的心肌细胞损伤。
Eur Rev Med Pharmacol Sci. 2019 Oct;23(20):9050-9057. doi: 10.26355/eurrev_201910_19307.
3
Long noncoding RNA DDX11-AS1 induced by YY1 accelerates colorectal cancer progression through targeting miR-873/CLDN7 axis.
通过机器学习识别肝细胞癌的程序性细胞死亡指数以改善预后和治疗反应：生物信息学分析和实验验证。
Front Immunol. 2023 Dec 19;14:1298290. doi: 10.3389/fimmu.2023.1298290. eCollection 2023.
4
METTL3-mediated m A methylation of TRAF5 inhibits lung adenocarcinoma cell metastasis via activation of the PI3K/AKT/NF-κB signaling pathway.METTL3 介导的 TRAF5 mA 甲基化通过激活 PI3K/AKT/NF-κB 信号通路抑制肺腺癌细胞转移。
Kaohsiung J Med Sci. 2024 Feb;40(2):150-160. doi: 10.1002/kjm2.12791. Epub 2023 Dec 13.
5
Silencing of TRAF5 enhances necroptosis in hepatocellular carcinoma by inhibiting LTBR-mediated NF-κB signaling.沉默 TRAF5 通过抑制 LTBR 介导的 NF-κB 信号通路增强肝癌细胞的坏死性凋亡。
PeerJ. 2023 Jun 22;11:e15551. doi: 10.7717/peerj.15551. eCollection 2023.
6
The Cuproptosis-Related Long Noncoding RNA Signature Predicts Prognosis and Immune Cell Infiltration in Hepatocellular Carcinoma.铜死亡相关长链非编码RNA特征预测肝细胞癌的预后和免疫细胞浸润
J Oncol. 2023 Feb 27;2023:9557690. doi: 10.1155/2023/9557690. eCollection 2023.
7
Long non-coding RNA DDX11-AS1 promotes the proliferation and migration of glioma cells by combining with HNRNPC.长链非编码RNA DDX11-AS1通过与HNRNPC结合促进胶质瘤细胞的增殖和迁移。
Mol Ther Nucleic Acids. 2022 Apr 26;28:601-612. doi: 10.1016/j.omtn.2022.04.016. eCollection 2022 Jun 14.
8
Correction to: Silenced lncRNA DDX11-AS1 or up-regulated microRNA-34a-3p inhibits malignant phenotypes of hepatocellular carcinoma cells via suppression of TRAF5.更正：沉默的长链非编码RNA DDX11-AS1或上调的微小RNA-34a-3p通过抑制TRAF5抑制肝癌细胞的恶性表型。
Cancer Cell Int. 2021 Dec 9;21(1):659. doi: 10.1186/s12935-021-02360-6.
长非编码 RNA DDX11-AS1 受 YY1 诱导通过靶向 miR-873/CLDN7 轴加速结直肠癌进展。
Eur Rev Med Pharmacol Sci. 2019 Jul;23(13):5714-5729. doi: 10.26355/eurrev_201907_18309.
4
Deregulation of miR-34a and Its Chaperon Hsp70 in Hepatitis C virus-Induced Liver Cirrhosis and Hepatocellular Carcinoma Patients.丙型肝炎病毒诱导的肝硬化和肝细胞癌患者中miR-34a及其伴侣蛋白Hsp70的失调
Asian Pac J Cancer Prev. 2017 Sep 27;18(9):2395-2401. doi: 10.22034/APJCP.2017.18.9.2395.