Glaucoma Center of Excellence, Wilmer Eye Institute, Johns Hopkins University School of Medicine, 600 North Wolfe Street, Maumenee B-110, Baltimore, MD, 21287, USA.
Glaucoma Center of Excellence, Wilmer Eye Institute, Johns Hopkins University School of Medicine, 600 North Wolfe Street, Maumenee B-110, Baltimore, MD, 21287, USA.
Exp Eye Res. 2021 May;206:108545. doi: 10.1016/j.exer.2021.108545. Epub 2021 Mar 20.
Basement membranes help to establish, maintain, and separate their associated tissues. They also provide growth and signaling substrates for nearby resident cells. The internal limiting membrane (ILM) is the basement membrane at the ocular vitreoretinal interface. While the ILM is essential for normal retinal development, it is dispensable in adulthood. Moreover, the ILM may constitute a significant barrier to emerging ocular therapeutics, such as viral gene therapy or stem cell transplantation. Here we take a neurodevelopmental perspective in examining how retinal neurons, glia, and vasculature interact with individual extracellular matrix constituents at the ILM. In addition, we review evidence that the ILM may impede novel ocular therapies and discuss approaches for achieving retinal parenchymal targeting of gene vectors and cell transplants delivered into the vitreous cavity by manipulating interactions with the ILM.
基膜有助于建立、维持和分隔其相关组织。它们还为附近的常驻细胞提供生长和信号底物。内界膜（ILM）是眼玻璃体视网膜界面的基膜。虽然 ILM 对正常视网膜发育至关重要，但在成年期是可有可无的。此外，ILM 可能成为新兴眼部治疗方法的重大障碍，例如病毒基因治疗或干细胞移植。在这里，我们从神经发育的角度研究了视网膜神经元、神经胶质和血管如何与 ILM 上的单个细胞外基质成分相互作用。此外，我们还回顾了 ILM 可能阻碍新型眼部治疗的证据，并讨论了通过操纵与 ILM 的相互作用来实现玻璃体腔中基因载体和细胞移植对视网膜实质靶向的方法。
