Khajei Sina, Mirnajafi-Zadeh Javad, Sheibani Vahid, Ahmadi-Zeidabadi Meysam, Masoumi-Ardakani Yaser, Rajizadeh Mohammad Amin, Esmaeilpour Khadijeh
Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.
Department of Physiology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Brain Res Bull. 2021 Jun;171:75-83. doi: 10.1016/j.brainresbull.2021.03.013. Epub 2021 Mar 19.
Kindling results in abnormal synaptic potentiation and significant impairment in learning and memory. Electromagnetic field (EMF) effects on learning and memory in kindled animals and its effects on hippocampal neural activity are largely unknown. In the current study, the effects of EMF on learning and memory, as well as hippocampal synaptic plasticity, in kindled rats were investigated. EMF (10 mT; 100 Hz) was applied to fully kindled animals one hour/day for a period of one week. The behavioral and electrophysiological studies were performed 24 h following the EMF application. The kindled rats showed spatial learning deficits during the training phase of the Morris water maze (MWM) test. Moreover, there were increments in escape latency and path length compared to the sham group. The kindled rats spent less time in the target-quadrant probe test, indicating spatial memory impairment. Applying EMF to the KEMF group (kindling + EMF) restored learning and memory, and decreased escape latency and path length significantly compared to the kindled group. EMF alone had no significant effects on the learning and memory parameters. Based on the open field (OF) test results, EMF alone in the EMF group, but not in the kindled or the KEMF groups, decreased the total traveled distance and increased the spent time in the peripheral zone, compared to the sham group. Based on electrophysiological results, applying EMF in the KEMF group returned the ability of synaptic potentiation to the hippocampal CA1 area and high-frequency stimulation induced long-term potentiation (LTP). Accordingly, EMF can be considered a potential therapy for seizure-induced deficits in learning and memory.
点燃效应会导致异常的突触增强以及学习和记忆的显著受损。电磁场（EMF）对点燃动物学习和记忆的影响及其对海马神经活动的影响在很大程度上尚不清楚。在当前研究中，研究了EMF对点燃大鼠学习和记忆以及海马突触可塑性的影响。将EMF（10 mT；100 Hz）每天施加于完全点燃的动物1小时，持续一周。在施加EMF后24小时进行行为和电生理研究。点燃大鼠在莫里斯水迷宫（MWM）测试的训练阶段表现出空间学习缺陷。此外，与假手术组相比，逃避潜伏期和路径长度增加。在目标象限探针测试中，点燃大鼠花费的时间较少，表明存在空间记忆损伤。对KEMF组（点燃+EMF）施加EMF可恢复学习和记忆，与点燃组相比，显著降低了逃避潜伏期和路径长度。单独的EMF对学习和记忆参数没有显著影响。根据旷场（OF）测试结果，与假手术组相比，EMF组单独的EMF（而非点燃组或KEMF组）减少了总行进距离，并增加了在外周区域花费的时间。根据电生理结果，在KEMF组中施加EMF恢复了海马CA1区的突触增强能力以及高频刺激诱导的长时程增强（LTP）。因此，EMF可被视为治疗癫痫引起的学习和记忆缺陷的一种潜在疗法。
