Department of Conservative Dentistry, College of Dentistry, University of Mosul, Mosul, Iraq.
Department of Dentistry, Ministry of Health and Environment, Nineveh Health Directorate, Al Salam Hospital, Mosul, Iraq.
Indian J Dent Res. 2020 Nov-Dec;31(6):846-851. doi: 10.4103/ijdr.IJDR_359_19.
Bacterial elimination from the root canal system is considered as an essential factor for a successful endodontic treatment.
To investigate both in vitro and in vivo efficacy of calcium hydroxide paste (CH) combined with 2% chlorhexidine gel (CHX) at various time intervals on Enterococcus faecalis (E.f) when used as an intracanal medicament (ICM).
For the in vitro study, 45 single-rooted teeth were used. After instrumentation, roots were infected with E.f and then divided into three groups (gps) (15 n/gp) according to the time interval for the medicament to be left inside the canal (1 week, 2 weeks and 3 weeks). ICM used was 2% CHX combined with CH (CH + CHX). At the end of each time interval, the antibacterial effect was evaluated using the agar diffusion test. For the in vivo study, 45 single-rooted teeth were selected. After sterilisation and access opening, the first pre-treatment sample was taken, then after instrumentation second post-instrumentation sample was obtained. Later, teeth were divided into 3 gps (15 n/gp) (1 week, 2 weeks and 3 weeks) according to the time interval for the medicament to be left inside the canal. ICM used was 2% CH + CHX. After each period, the third post-medication sample was taken. ANOVA and Tukey post hoc tests were used for statistical analysis.
Both in vitro and in vivo studies have found that 2% of CH + CHX had a significant antibacterial effect for 1 week and 2 weeks. However, the antibacterial effect was significantly decreased after 3 weeks.
CH + CHX can be used successfully for the reduction of E.f for 14 days when used as ICM.
从根管系统中消除细菌被认为是根管治疗成功的关键因素。
研究氢氧化钙糊剂（CH）联合 2%洗必泰凝胶（CHX）在不同时间间隔作为根管内药物（ICM）应用于粪肠球菌（E.f）时的体外和体内疗效。
在体外研究中，使用了 45 颗单根牙。器械预备后，用 E.f 感染牙根，然后根据药物在根管内停留的时间间隔将牙根分为三组（每组 15 个）（1 周、2 周和 3 周）。所用的 ICM 是 2% CHX 联合 CH（CH + CHX）。在每个时间间隔结束时，使用琼脂扩散试验评估抗菌效果。在体内研究中，选择了 45 颗单根牙。经过消毒和开口后，取第一次预处理样本，然后在器械预备后取第二次样本。之后，根据药物在根管内停留的时间间隔，将牙齿分为三组（每组 15 个）（1 周、2 周和 3 周）。所用的 ICM 是 2% CH + CHX。每个时期后，取第三个用药后样本。采用方差分析和 Tukey 事后检验进行统计学分析。
体外和体内研究均发现，2%的 CH + CHX 在 1 周和 2 周时具有显著的抗菌效果。然而，在 3 周后，抗菌效果显著降低。
CH + CHX 可成功用作 ICM，在 14 天内减少 E.f。
