免疫组化染色显示全细胞角蛋白阳性的肿瘤芽和细胞质假碎片是口腔鳞状细胞癌区域淋巴结转移风险评估的可靠早期预测变量。

Pancytokeratin immunostained tumor buds and cytoplasmic pseudofragments are reliable early predictive variables for regional lymph node metastatic risk assessment of oral squamous cell carcinoma.

机构信息

Department of Oral Pathology and Microbiology, Faculty of Dental Sciences, M.S. Ramaiah University of Applied Sciences, Bangalore, Karnataka, India.

Department of Oral and Maxillofacial Surgery, Faculty of Dental Sciences, M.S. Ramaiah University of Applied Sciences, Bangalore, Karnataka, India.

出版信息

Indian J Dent Res. 2020 Nov-Dec;31(6):904-910. doi: 10.4103/ijdr.IJDR_101_19.

DOI:10.4103/ijdr.IJDR_101_19

PMID:33753662

Abstract

CONTEXT

The 5-year survival rate in patients with cervical lymph node metastasis is reported to be 20-36% post surgery as compared to 63-86% in patients without nodal involvement. This necessitates assessing the metastatic potential of OSCC patients.

OBJECTIVES

To evaluate the role of Pancytokeratin immunostained tumour buds and cytoplasmic pseudofragments with other histopathological and immunohistochemical variables in predicting metastatic risk of Oral Squamous Cell Carcinoma (OSCC).

SETTINGS AND DESIGN

Retrospective study on archival tissues of OSCC available from the Department of Oral Pathology and Microbiology.

METHODOLOGY

Totally.

UNLABELLED

40 samples of 117 histopathologically diagnosed OSCC samples were selected that displayed metastatic risk variables like invasive tumour front pattern, lymphovascular invasion, tumour buds and cytoplasmic pseudofragmentation and were grouped into 20 each of metastatic and non-metastatic OSCC. 5 normal oral mucosa samples were included in the control group. The 45 tissues were stained with congo red to assess tumour - associated tissue eosinophilia (TATE) and immunohistochemically evaluated for tumour budding and cytoplasmic pseudofragmentation using pancytokeratin, proliferation (Ki-67), microvessel density (MVD)(CD31) and lymphatic vessel density (LVD)( LYVE-1).

STATISTICAL ANALYSIS

Pearson's Chi square test and Man Whitney U test were used and analysed by Statistical Package for the Social Sciences (SPSS) software version 20.0.

RESULTS

Metastatic OSCC showed significantly high number of tumour buds (p = 0.001), cytoplasmic pseudofragments (p = 0.008), higher tumour grade (p = 0.038), lymphovascular invasion (p = 0.008) and LVD (p = 0.013), aggressive invasive tumour front pattern (p = 0.001) compared to non-metastatic OSCC.

CONCLUSION

Pancytokeratin immunostained tumour buds, cytoplasmic pseudofragments and higher LYVE-1 expression may be used as independent predictors for OSCC metastasis.

UNLABELLED

This study highlights the importance of recognizing the early metastatic risk variables that navigates the surgeon in planning appropriate therapy for OSCC.

摘要

背景

与无淋巴结受累的患者（63-86%）相比，有颈淋巴结转移的患者术后 5 年生存率报道为 20-36%。这就需要评估口腔鳞状细胞癌（OSCC）患者的转移潜能。

目的

评估全角蛋白免疫染色肿瘤芽和细胞质假碎片与其他组织病理学和免疫组织化学变量在预测口腔鳞状细胞癌（OSCC）转移风险中的作用。

设置和设计

回顾性研究来自口腔病理学和微生物学系的存档 OSCC 组织。

方法

总共选择了 117 例组织病理学诊断为 OSCC 的样本中的 40 个样本，这些样本显示出转移性风险变量，如侵袭性肿瘤前缘模式、淋巴管血管侵犯、肿瘤芽和细胞质假片段，并将其分为转移性和非转移性 OSCC 各 20 个。纳入 5 例正常口腔黏膜样本作为对照组。用刚果红染色 45 个组织，评估肿瘤相关组织嗜酸性粒细胞（TATE），并用全角蛋白、增殖（Ki-67）、微血管密度（MVD）（CD31）和淋巴管密度（LVD）（LYVE-1）对肿瘤芽和细胞质假片段进行免疫组织化学评估。