Okuyama Kohei, Fukushima Hiromasa, Naruse Tomofumi, Yanamoto Souichi, Tsuchihashi Hiroki, Umeda Masahiro
Department of Clinical Oral Oncology, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1, Sakamoto, Nagasaki-shi, Nagasaki, 852-8588, Japan.
Nagasaki University School of Dentistry, Nagasaki, Japan.
Pathol Oncol Res. 2019 Apr;25(2):603-609. doi: 10.1007/s12253-018-0529-y. Epub 2018 Nov 1.
Oral squamous cell carcinoma (OSCC) with invasion into the mandibular medullary space has been reported to be a predictive factor for cervical lymph node metastasis (CLNM). As CLNM has been associated with the stemness of cancer cells, we aimed to evaluate the relationship between clinical characteristics and immunohistochemical findings on the invasion front of the medullary invasive OSCC and CLNM. The medical records of 25 patients with the mandibular medullary invasive OSCC who were performed mandibulectomy and neck dissection in our department from 2010 to 2016 were examined. Serial sections were stained with antibodies against CD44 variant 6 (CD44v6) to examine cancer stemness and to evaluate the number of tumor buds in the medullary invasion front of the mandibular invasive OSCC. Categorical data were analyzed by Fisher's exact test. The expression of CD44v6 and the number of tumor buds between the groups with and without pathological CLNM (CLNM+ and CLNM-, respectively) were analyzed using the Mann-Whitney U test. Of the 25 patients, 11 patients were CLNM+. Of the several measured variables, histologic differentiation of the mandibular invasive OSCC was a significant factor for CLNM+. CD44v6 expression and tumor bud formation in the medullary invasion front of the mandibular invasive OSCC were significantly higher in the CLNM+ group, suggests that both CD44v6 and tumor budding in the medullary invasion front are predictive factors for CLNM.
据报道，侵犯下颌骨骨髓腔的口腔鳞状细胞癌（OSCC）是颈部淋巴结转移（CLNM）的一个预测因素。由于CLNM与癌细胞的干性有关，我们旨在评估骨髓浸润性OSCC侵犯前沿的临床特征与免疫组化结果之间的关系，并分析其与CLNM的相关性。我们查阅了2010年至2016年在我科接受下颌骨切除术和颈部清扫术的25例下颌骨骨髓浸润性OSCC患者的病历。连续切片用抗CD44变体6（CD44v6）抗体染色，以检测癌干细胞特性，并评估下颌骨浸润性OSCC骨髓侵犯前沿的肿瘤芽数量。分类数据采用Fisher精确检验进行分析。采用Mann-Whitney U检验分析有或无病理性CLNM（分别为CLNM+和CLNM-）组之间CD44v6的表达和肿瘤芽数量。在这25例患者中，11例有CLNM+。在几个测量变量中，下颌骨浸润性OSCC的组织学分化是CLNM+的一个重要因素。CLNM+组下颌骨浸润性OSCC骨髓侵犯前沿的CD44v6表达和肿瘤芽形成明显更高，这表明骨髓侵犯前沿的CD44v6和肿瘤芽形成都是CLNM的预测因素。
