State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, College of Chemistry, Jilin University, Changchun 130012, People's Republic of China.
School of Pharmaceutical Sciences, Key Laboratory of Innovative Drug Development and Evaluation, Hebei Medical University, Shijiazhuang, 050017, China.
Theranostics. 2021 Mar 4;11(10):4872-4893. doi: 10.7150/thno.56409. eCollection 2021.
Organelles are involved in many cell life activities, and their metabolic or functional disorders are closely related to apoptosis, neurodegenerative diseases, cardiovascular diseases, and the development and metastasis of cancers. The explorations of subcellular structures, microenvironments, and their abnormal conditions are conducive to a deeper understanding of many pathological mechanisms, which are expected to achieve the early diagnosis and the effective therapy of diseases. Organelles are also the targeted locations of drugs, and they play significant roles in many targeting therapeutic strategies. Surface-enhanced Raman spectroscopy (SERS) is a powerful analytical tool that can provide the molecular fingerprint information of subcellular compartments and the real-time cellular dynamics in a non-invasive and non-destructive way. This review aims to summarize the recent advances of SERS studies on subcellular compartments, including five parts. The introductions of SERS and subcellular compartments are given. SERS is promising in subcellular compartment studies due to its molecular specificity and high sensitivity, and both of which highly match the high demands of cellular/subcellular investigations. Intracellular SERS is mainly cataloged as the labeling and label-free methods. For subcellular targeted detections and therapies, how to internalize plasmonic nanoparticles or nanostructure in the target locations is a key point. The subcellular compartment SERS detections, SERS measurements of isolated organelles, investigations of therapeutic mechanisms from subcellular compartments and microenvironments, and integration of SERS diagnosis and treatment are sequentially presented. A perspective view of the subcellular SERS studies is discussed from six aspects. This review provides a comprehensive overview of SERS applications in subcellular compartment researches, which will be a useful reference for designing the SERS-involved therapeutic systems.
细胞器参与许多细胞生命活动，其代谢或功能障碍与细胞凋亡、神经退行性疾病、心血管疾病以及癌症的发生和转移密切相关。对亚细胞结构、微环境及其异常状态的探索有助于更深入地了解许多病理机制，有望实现疾病的早期诊断和有效治疗。细胞器也是药物的靶向部位，在许多靶向治疗策略中发挥着重要作用。表面增强拉曼光谱（SERS）是一种强大的分析工具，可提供亚细胞区室的分子指纹信息和实时细胞动力学，具有非侵入性和非破坏性。本综述旨在总结 SERS 对亚细胞区室研究的最新进展，包括五个部分。介绍了 SERS 和亚细胞区室。由于 SERS 具有分子特异性和高灵敏度，非常符合细胞/亚细胞研究的高要求，因此在亚细胞区室研究中具有广阔的应用前景。细胞内 SERS 主要分为标记和无标记方法。对于亚细胞靶向检测和治疗，如何将等离子体纳米粒子或纳米结构内化到目标位置是一个关键点。依次介绍了亚细胞区室 SERS 检测、分离细胞器的 SERS 测量、亚细胞区室和微环境的治疗机制研究以及 SERS 诊断和治疗的整合。从六个方面讨论了亚细胞 SERS 研究的观点。本综述全面概述了 SERS 在亚细胞区室研究中的应用，为设计涉及 SERS 的治疗系统提供了有益的参考。
Zotero 插件
