Department of Pathophysiology, Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, State Key Laboratory of Oncogenes and Related Genes and Chinese Academy of Medical Sciences Research Unit (NO.2019RU043), Shanghai Cancer Institute, Rui-Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.
Sci China Life Sci. 2021 Nov;64(11):1858-1867. doi: 10.1007/s11427-020-1895-2. Epub 2021 Mar 18.
Long non-coding RNAs (lncRNAs) are widely involved in a variety of biological processes, including epithelial-mesenchymal transition (EMT). In the current study, we found that lncRNA small nucleolar RNA host gene 8 (SNHG8) was tightly correlated with EMT-associated gene signatures, and was down-regulated by Zinc finger E-box-binding homeobox 1 (ZEB1) during EMT progress. Functionally, knockdown of SNHG8 induced EMT in epithelial cells, through destabilizing the CDH1 mRNA dependent on a 17-nucleotide sequence shared by SNHG8 and CDH1. In addition, analysis with public database showed that SNHG8 tended to be down-regulated in different cancer types and the lower expression of SNHG8 predicted poorer prognosis. Taken together, our study reports a ZEB1-repressed lncRNA SNHG8 which is important for stabilizing CDH1 mRNA, thereby maintaining the epithelial status of epithelial cells.
长链非编码 RNA（lncRNA）广泛参与多种生物学过程，包括上皮-间充质转化（EMT）。在本研究中，我们发现 lncRNA 小核仁 RNA 宿主基因 8（SNHG8）与 EMT 相关基因特征密切相关，并且在 EMT 进展过程中被锌指 E 盒结合同源盒 1（ZEB1）下调。功能上，SNHG8 的敲低诱导上皮细胞发生 EMT，通过破坏 CDH1 mRNA 的稳定性，而该 CDH1 mRNA 依赖于 SNHG8 和 CDH1 之间共享的 17 个核苷酸序列。此外，通过公共数据库分析表明，SNHG8 在不同癌症类型中倾向于下调，并且 SNHG8 的低表达预示着预后较差。综上所述，我们的研究报告了一种 ZEB1 抑制的 lncRNA SNHG8，它对于稳定 CDH1 mRNA 非常重要，从而维持上皮细胞的上皮状态。
