Molecular Physiology Laboratory, Department of Biochemistry, Bharathiar University, Coimbatore, Tamil Nadu, 641046, India.
Department of Biotechnology, School of Biotechnology and Genetic Engineering, Bharathiar University, Coimbatore, Tamil Nadu, 641046, India.
Mol Nutr Food Res. 2021 May;65(10):e2001224. doi: 10.1002/mnfr.202001224. Epub 2021 Apr 16.
Occurrence of obesity and its associated metabolic disorders continues to escalate. The present study evaluates the anti-obesity effects of ethanolic fruit extract of Terminalia chebula (EETC) on high fat diet induced obese mice. The bioactive compounds present in the EETC is evaluated by Fourier-transform infrared (FT-IR), Gas chromatography-mass spectrometry (GC-MS), and Liquid chromatography-mass spectrometry (LC-MS) analysis. The effects of EETC on energy intake, glucose tolerance, and various biochemical parameters were analyzed using laboratory mice. Relative gene expression of Fatty acid synthase (FAS), Peroxisome proliferator-activated receptors α (PPARα), Carnitine palmitoyltransferase-1 (CPT-1), Tumor necrosis factor alpha (TNF-α) as well as Interleukin 6 (IL-6) were analyzed in liver and adipose tissues. The findings reveal the hypolipidemic and anti-obesity potential of EETC on high fat fed obese mice. EETC exerts its anti-obesity effects by suppressing lipogenesis through reduction in lipogenic enzyme (FAS) expression, increased fatty acid oxidation via PPARα and CPT-1 and by triggering the anti-inflammatory responses. To our knowledge, this is the first report of the effect of EETC on PPARα and CPT-1 in in vivo.
肥胖及其相关代谢紊乱的发生率持续上升。本研究评估了诃子乙醇水果提取物（EETC）对高脂肪饮食诱导肥胖小鼠的抗肥胖作用。通过傅里叶变换红外（FT-IR）、气相色谱-质谱（GC-MS）和液相色谱-质谱（LC-MS）分析评估 EETC 中的生物活性化合物。使用实验室小鼠分析 EETC 对能量摄入、葡萄糖耐量和各种生化参数的影响。分析肝脏和脂肪组织中脂肪酸合酶（FAS）、过氧化物酶体增殖物激活受体α（PPARα）、肉碱棕榈酰转移酶-1（CPT-1）、肿瘤坏死因子α（TNF-α）和白细胞介素 6（IL-6）的相对基因表达。研究结果表明，EETC 对高脂肪喂养肥胖小鼠具有降血脂和抗肥胖作用。EETC 通过降低脂肪生成酶（FAS）的表达来抑制脂肪生成，通过 PPARα 和 CPT-1 增加脂肪酸氧化，并通过触发抗炎反应来发挥其抗肥胖作用。据我们所知，这是 EETC 对体内 PPARα 和 CPT-1 影响的首次报道。
