Platelet Proteomics Group, Center for Research in Molecular Medicine and Chronic Diseases (CIMUS), Universidade de Santiago de Compostela, and Instituto de Investigación Sanitaria de Santiago de Compostela (IDIS), Santiago de Compostela, Spain.
Proteomics. 2021 Jul;21(13-14):e2000089. doi: 10.1002/pmic.202000089. Epub 2021 May 4.
In transfusion centres, blood components are divided and stored following specific guidelines. The storage temperature and time vary among the blood cells but all of them release extracellular vesicles (EVs) under blood bank conditions. The clinical impact of such vesicles in blood components for transfusion is an object of debate, but should be considered and is being investigated. In this context, proteomics is an excellent tool to study the cargo and composition of EVs derived from red blood cells and platelets, since such vesicles are enriched in lipids and proteins. The development of quantitative mass spectrometry techniques and the evolution of bioinformatics have allowed the identification of novel EVs biomarkers for different diseases. In this context, the application of high coverage proteomic tools to the analysis of EVs in the transfusion medicine field would provide information about storage lesions and possible transfusion adverse reactions. This viewpoint article approaches the potential of proteomics to investigate the impact of EVs in blood bank transfusion components, especially red blood cells and platelets.
在输血中心，血液成分会根据特定的指导方针进行分离和储存。不同的血细胞有不同的储存温度和时间，但在血库条件下，所有的血细胞都会释放细胞外囊泡（EVs）。这些囊泡在输血用血液成分中的临床影响是一个有争议的问题，但应该被考虑并正在研究中。在这种情况下，蛋白质组学是研究来源于红细胞和血小板的 EVs 货物和组成的极好工具，因为这些囊泡富含脂质和蛋白质。定量质谱技术的发展和生物信息学的演进使得不同疾病的新型 EVs 生物标志物的鉴定成为可能。在这种情况下，将高覆盖率蛋白质组学工具应用于输血医学领域中 EVs 的分析，将提供关于储存损伤和可能的输血不良反应的信息。这篇观点文章探讨了蛋白质组学在研究 EVs 对血库输血成分（尤其是红细胞和血小板）的影响方面的潜力。
