Department of Cardiothoracic Surgery, The First Hospital of Jiaxing (Affiliated Hospital of Jiaxing University), Jiaxing, Zhejiang China.
Technol Cancer Res Treat. 2021 Jan-Dec;20:1533033821990036. doi: 10.1177/1533033821990036.
miR-301b-3p is reported in various human cancers for its abnormal expression, while the role and molecular mechanisms in lung adenocarcinoma (LUAD) remain unclear, and this is the focus of the present study.
TCGA database was consulted to know gene expression in LUAD tissue. CCK-8, colony formation assay and Transwell assay were applied to identify the role of target genes in regulating LUAD cell biological properties. Bioinformatics analysis plus dual-luciferase assay were performed to validate the potential connection between genes.
miR-301b-3p and DLC1 were the target genes of this study and respectively differentially up-regulated and down-regulated in LUAD. Functional experiments indicated that miR-301b-3p contributed to cancer cell proliferation, migration and invasion, while this effect was reversed with overexpressed DLC1 which was identified as a direct target of and regulated by miR-301b-3p.
Collectively, miR-301b-3p was identified to actively function on LUAD malignant progression by suppressing DLC1 expression. This discovery provides a novel therapeutic strategy for LUAD patients, which helps improve the survival of patients.
miR-301b-3p 在各种人类癌症中因其异常表达而被报道，而其在肺腺癌（LUAD）中的作用和分子机制尚不清楚，这也是本研究的重点。
查阅 TCGA 数据库以了解 LUAD 组织中的基因表达。CCK-8、集落形成实验和 Transwell 实验用于鉴定靶基因在调节 LUAD 细胞生物学特性中的作用。生物信息学分析加双荧光素酶报告基因实验验证基因之间的潜在联系。
miR-301b-3p 和 DLC1 是本研究的靶基因，在 LUAD 中分别差异上调和下调。功能实验表明，miR-301b-3p 促进癌细胞的增殖、迁移和侵袭，而过表达的 DLC1 则逆转了这一效应，DLC1 被鉴定为 miR-301b-3p 的直接靶基因，并受其调控。
总之，miR-301b-3p 通过抑制 DLC1 的表达，积极作用于 LUAD 的恶性进展。这一发现为 LUAD 患者提供了一种新的治疗策略，有助于提高患者的生存率。
