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microRNA-140-3p 通过靶向 TYMS（胸苷酸合成酶）抑制肺腺癌细胞的增殖、迁移、侵袭和血管生成。

MicroRNA-140-3p represses the proliferation, migration, invasion and angiogenesis of lung adenocarcinoma cells via targeting TYMS (thymidylate synthetase).

机构信息

No.1 Department of Respiratory and Critical Care Medicine, Cangzhou Central Hospital, No. 16, Xinhua West Road, Yunhe District, Cangzhou City, Hebei Province, China.

Department of No. 1 Pediatrics, Cangzhou Hospital of Integrated TCM-WM, Cangzhou City, Hebei Province, China.

出版信息

Bioengineered. 2021 Dec;12(2):11959-11977. doi: 10.1080/21655979.2021.2009422.

DOI:10.1080/21655979.2021.2009422
PMID:34818974
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8810165/
Abstract

MicroRNA (miR)-140-3p has been proved to repress lung adenocarcinoma (LUAD), and our study aims to further evaluate the mechanism. Bioinformatic analyses were performed. The viability, proliferation, migration, invasion and angiogenesis of transfected LUAD cells were all determined via Cell Counting Kit-8, colony formation, Scratch, Transwell, and tube formation assays. The targeting relationship between miR-140-3p and thymidylate synthetase (TYMS) was confirmed by dual-luciferase reporter assay. Relative expressions of miR-140-3p, TYMS, epithelial-to-mesenchymal transition- (E-cadherin, N-cadherin, vimentin), angiogenesis- (vascular endothelial growth factor (VEGF)), and apoptosis-related factors (cleaved caspase-3, B-cell lymphoma-2 (Bcl-2), Bcl-2-associated X protein (Bax)) were quantified by quantitative real-time polymerase chain reaction or Western blot. TYMS was high-expressed yet miR-140-3p was low-expressed in LUAD cells. Upregulation of miR-140-3p inhibited TYMS expression, viability, colony formation, migration, invasion, and tube length within LUAD cells, while downregulation of miR-140-3p did oppositely. Silenced TYMS, the downstream target gene of miR-140-3p, reversed the effects of miR-140-3p downregulation on TYMS expression, cell viability, colony formation, migration, invasion, and tube length as well as the metastasis-, apoptosis- and angiogenesis-related proteins in LUAD cells. Upregulation of miR-140-3p inhibited the proliferation, migration, invasion and angiogenesis of LUAD cells via targeting TYMS.

摘要

微小 RNA（miR）-140-3p 已被证明可抑制肺腺癌（LUAD），我们的研究旨在进一步评估其机制。进行了生物信息学分析。通过细胞计数试剂盒-8、集落形成、划痕、Transwell 和管形成测定来确定转染的 LUAD 细胞的活力、增殖、迁移、侵袭和血管生成。通过双荧光素酶报告基因测定证实了 miR-140-3p 和胸苷酸合成酶（TYMS）之间的靶向关系。通过定量实时聚合酶链反应或 Western blot 定量 miR-140-3p、TYMS、上皮-间充质转化（E-cadherin、N-cadherin、vimentin）、血管生成（血管内皮生长因子（VEGF））和凋亡相关因子（cleaved caspase-3、B 细胞淋巴瘤-2（Bcl-2）、Bcl-2 相关 X 蛋白（Bax））的相对表达。在 LUAD 细胞中，TYMS 高表达而 miR-140-3p 低表达。上调 miR-140-3p 抑制 TYMS 表达、LUAD 细胞活力、集落形成、迁移、侵袭和管长度，而下调 miR-140-3p 则相反。沉默 TYMS，miR-140-3p 的下游靶基因，逆转了 miR-140-3p 下调对 TYMS 表达、细胞活力、集落形成、迁移、侵袭和管长度以及 LUAD 细胞中转移、凋亡和血管生成相关蛋白的影响。上调 miR-140-3p 通过靶向 TYMS 抑制 LUAD 细胞的增殖、迁移、侵袭和血管生成。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f42/8810165/9cc44ffcaebd/KBIE_A_2009422_F0010_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f42/8810165/50878a7f316f/KBIE_A_2009422_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f42/8810165/a8f7d364bf83/KBIE_A_2009422_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f42/8810165/7cccc5a86226/KBIE_A_2009422_F0003_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f42/8810165/d4c0cca536f2/KBIE_A_2009422_F0004_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f42/8810165/4f0cf6a6c4c1/KBIE_A_2009422_F0005_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f42/8810165/a8ab2af278c4/KBIE_A_2009422_F0006_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f42/8810165/523bdd08090a/KBIE_A_2009422_F0007_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f42/8810165/4aebba4e5a86/KBIE_A_2009422_F0008_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f42/8810165/f5810382893b/KBIE_A_2009422_F0009_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f42/8810165/9cc44ffcaebd/KBIE_A_2009422_F0010_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f42/8810165/50878a7f316f/KBIE_A_2009422_F0001_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f42/8810165/a8f7d364bf83/KBIE_A_2009422_F0002_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f42/8810165/7cccc5a86226/KBIE_A_2009422_F0003_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f42/8810165/d4c0cca536f2/KBIE_A_2009422_F0004_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f42/8810165/4f0cf6a6c4c1/KBIE_A_2009422_F0005_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f42/8810165/a8ab2af278c4/KBIE_A_2009422_F0006_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f42/8810165/523bdd08090a/KBIE_A_2009422_F0007_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f42/8810165/4aebba4e5a86/KBIE_A_2009422_F0008_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f42/8810165/f5810382893b/KBIE_A_2009422_F0009_OC.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1f42/8810165/9cc44ffcaebd/KBIE_A_2009422_F0010_OC.jpg

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[4]
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[5]
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Naunyn Schmiedebergs Arch Pharmacol. 2024-8

[6]
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[7]
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本文引用的文献

[1]
Circular RNA circ_0008274 upregulates granulin to promote the progression of hepatocellular carcinoma via sponging microRNA -140-3p.

Bioengineered. 2021-12

[2]
Establishment and validation of a prognostic signature for lung adenocarcinoma based on metabolism-related genes.

Cancer Cell Int. 2021-4-15

[3]
Crosstalk between miRNAs and signaling pathways involved in pancreatic cancer and pancreatic ductal adenocarcinoma.

Eur J Pharmacol. 2021-6-15

[4]
Circ-AASDH functions as the progression of early stage lung adenocarcinoma by targeting miR-140-3p to activate E2F7 expression.

Transl Lung Cancer Res. 2021-1

[5]
A Low-Cost Nanomaterial-based Electrochemical Immunosensor on Paper for High-Sensitivity Early Detection of Pancreatic Cancer.

Sens Actuators B Chem. 2020-2-15

[6]
miR-140-3p enhances cisplatin sensitivity and attenuates stem cell-like properties through repressing Wnt/β-catenin signaling in lung adenocarcinoma cells.

Exp Ther Med. 2020-8

[7]
CircRNA SMARCC1 Sponges MiR-140-3p to Regulate Cell Progression in Colorectal Cancer.

Cancer Manag Res. 2020-6-23

[8]
Boron Attenuates Heat Stress-Induced Apoptosis by Inhibiting Endoplasmic Reticulum Stress in Mouse Granulosa Cells.

Biol Trace Elem Res. 2021-2

[9]
miR-140-3p inhibits progression of non-small cell lung cancer by targeting Janus kinase 1.

J Biosci. 2020

[10]
Knockdown of circAPLP2 Inhibits Progression of Colorectal Cancer by Regulating miR-485-5p/FOXK1 Axis.

Cancer Biother Radiopharm. 2021-11

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