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通过使用样本沉淀法提高在精液中检测埃博拉病毒RNA的可能性。

Increased Likelihood of Detecting Ebola Virus RNA in Semen by Using Sample Pelleting.

作者信息

Bozman Courtney M, Fallah Mosoka, Sneller Michael C, Freeman Catherine, Fakoli Lawrence S, Shobayo Bode I, Dighero-Kemp Bonnie, Reilly Cavan S, Kuhn Jens H, Bolay Fatorma, Higgs Elizabeth, Hensley Lisa E

出版信息

Emerg Infect Dis. 2021 Apr;27(4):1239-1241. doi: 10.3201/eid2704.204175.

DOI:10.3201/eid2704.204175
PMID:33755000
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8007310/
Abstract

Ebola virus RNA can reside for months or years in semen of survivors of Ebola virus disease and is probably associated with increased risk for cryptic sexual transmission of the virus. A modified protocol resulted in increased detection of Ebola virus RNA in semen and improved disease surveillance.

摘要

埃博拉病毒RNA可在埃博拉病毒病幸存者的精液中存留数月或数年，可能与该病毒隐秘性传播风险增加有关。一种改良方案提高了精液中埃博拉病毒RNA的检测率，并改善了疾病监测。

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本文引用的文献

1
A 40-Month Follow-Up of Ebola Virus Disease Survivors in Guinea (PostEbogui) Reveals Long-Term Detection of Ebola Viral Ribonucleic Acid in Semen and Breast Milk.几内亚埃博拉病毒病幸存者40个月随访（PostEbogui）显示，精液和母乳中长期检测到埃博拉病毒核糖核酸。
Open Forum Infect Dis. 2019 Nov 8;6(12):ofz482. doi: 10.1093/ofid/ofz482. eCollection 2019 Dec.
2
Ebola virus disease.埃博拉病毒病。
Nat Rev Dis Primers. 2020 Feb 20;6(1):13. doi: 10.1038/s41572-020-0147-3.
3
A Longitudinal Study of Ebola Sequelae in Liberia.利比里亚埃博拉后遗症的纵向研究。
N Engl J Med. 2019 Mar 7;380(10):924-934. doi: 10.1056/NEJMoa1805435.
4
Persistence and Sexual Transmission of Filoviruses.丝状病毒的持久性和性传播。
Viruses. 2018 Dec 2;10(12):683. doi: 10.3390/v10120683.
5
A day-by-day chronicle of the 2013-2016 Ebola outbreak.
Med Confl Surviv. 2018 Aug 3:1-2. doi: 10.1080/13623699.2018.1505366.
6
What was the effect of the West African Ebola outbreak on health programme performance, and did programmes recover?西非埃博拉疫情对卫生项目的实施产生了怎样的影响，这些项目是否恢复了？
Public Health Action. 2017 Jun 21;7(Suppl 1):S1-S2. doi: 10.5588/pha.17.0029.
7
Assessment and Optimization of the GeneXpert Diagnostic Platform for Detection of Ebola Virus RNA in Seminal Fluid.用于检测精液中埃博拉病毒RNA的GeneXpert诊断平台的评估与优化
J Infect Dis. 2017 Feb 15;215(4):547-553. doi: 10.1093/infdis/jiw599.
8
Validation of the Cepheid GeneXpert for Detecting Ebola Virus in Semen.赛沛GeneXpert检测精液中埃博拉病毒的验证
J Infect Dis. 2017 Feb 1;215(3):344-350. doi: 10.1093/infdis/jiw562.