Sasai K, Arakawa A
Department of Veterinary Medicine, College of Agriculture, University of Osaka Prefecture, Japan.
Res Vet Sci. 1988 Jan;44(1):1-5.
Colons from chickens, four weeks old, can best be maintained for 48 hours in a serum-free organ culture system using Trowell T8 medium-agar sheet at 25 degrees C. As determined by light microscopy as well as scanning and transmission electron microscopies, mucosal architecture involving classical ultrastructure of chicken colonic mucosa was preserved. Based on the morphological findings, it became evident that this organ culture system using Trowell T8 medium at 25 degrees C can be successfully used as an in vitro experimental model for as long as 48 hours. The organ culture system could be a useful tool in investigating mucosal function and mucosal response to pathogens.
四周龄鸡的结肠在使用Trowell T8培养基琼脂片的无血清器官培养系统中，于25摄氏度下可最佳维持48小时。通过光学显微镜以及扫描和透射电子显微镜观察确定，涉及鸡结肠黏膜经典超微结构的黏膜结构得以保留。基于形态学发现，很明显，这种在25摄氏度下使用Trowell T8培养基的器官培养系统可成功用作长达48小时的体外实验模型。该器官培养系统可能是研究黏膜功能和黏膜对病原体反应的有用工具。
