Shields H M, Yedlin S T, Bair F A, Goodwin C L, Alpers D H
Am J Anat. 1979 Jul;155(3):375-89. doi: 10.1002/aja.1001550307.
Ileum from rats 4, 9, 11, 12, and 15 days old can best be maintained for 24 hours in a system using Hanks' Balanced Salt Solution without fetal calf serum, at 25 degrees C and 21% O2. Suckling rat duodenum and jejunum were difficult to maintain well for 24 hours in this system or a variety of other systems that were tried. A temperature of 37 degrees C hastened deterioration of duodenum, jejunum or ileum. With ileum, 3H-thymidine and 14C-leucine were increasingly incorporated into DNA and protein over the 24-hour period. Light microscopy, as well as scanning and transmission electron microscopy, showed very good preservation of the ileum after 24 hours. The addition to the medium of hydrocortisone, 1 micron, and thyroxine, 0.01 micron, alone or in combination, did not change DNA or protein synthesis, or morphology, possibly because of the relatively short (24 hour) time period. Our organ culture system emphasizes the differences between suckling rat ileum and the rest of the intestine, and provides a new tool for evaluating, over a 24-hour period, the developing rat small intestine.
4日龄、9日龄、11日龄、12日龄和15日龄大鼠的回肠，在使用不含胎牛血清的汉克斯平衡盐溶液的系统中，于25摄氏度和21%氧气条件下，最适合维持24小时。在该系统或其他多种尝试过的系统中，乳鼠十二指肠和空肠很难良好维持24小时。37摄氏度的温度会加速十二指肠、空肠或回肠的退化。对于回肠，在24小时内，3H-胸腺嘧啶核苷和14C-亮氨酸越来越多地掺入DNA和蛋白质中。光学显微镜以及扫描和透射电子显微镜显示，24小时后回肠保存良好。单独或联合向培养基中添加1微克氢化可的松和0.01微克甲状腺素，不会改变DNA或蛋白质合成，也不会改变形态，这可能是因为时间相对较短（24小时）。我们的器官培养系统强调了乳鼠回肠与肠道其他部分之间的差异，并提供了一种在24小时内评估发育中大鼠小肠的新工具。
