Department of Civil and Mechanical Engineering, University of Cassino and Southern Lazio, via Gaetano di Biasio 43, 03043 Cassino, Italy.
Department of Civil, Architectural and Environmental Engineering, University of Naples Federico II, via Claudio 21, 80125 Naples, Italy; Department of Civil, Environmental, Land, Building Engineering and Chemistry, Polytechnic University of Bari, Via E. Orabona 4, 70125 Bari, Italy.
Bioresour Technol. 2021 Jun;330:124958. doi: 10.1016/j.biortech.2021.124958. Epub 2021 Mar 11.
This study investigated the feasibility of coupling simultaneous partial nitrification and denitrification (SPND) to biological phosphorus removal in continuous-flow intermittently-aerated moving bed biofilm reactors (MBBRs) fed with different carbon sources, i.e. ethanol and acetate. Bacterial cultivation at pH 8.2 (±0.2), 26-28 °C and SRT of 4 day and microaerobic/aerobic MBBR operation allowed to achieve average dissolved organic carbon (DOC), total inorganic nitrogen (TIN) and P-PO removal efficiencies (REs) of 100%, 81-88% and 83-86% at HRT of 1 day, dissolved oxygen (DO) range of 0.2-3 mg L and feed C/N and C/P ratios of 3.6 and 11, respectively. Acetate supplementation favored a diversified microbial community, while overgrowth of heterotrophs was observed when increasing feed C/N ratio in ethanol-fed MBBR. Illumina sequencing displayed the presence of putative phosphorus accumulating organisms (PAOs) such as Hydrogenophaga and Pseudomonas in MBBR biofilm and suspended biomass, whereas no typical NOB was identified during the study.
本研究考察了在连续流间歇曝气移动床生物膜反应器（MBBR）中耦合同时硝化反硝化（SPND）和生物除磷的可行性，该反应器采用不同的碳源，即乙醇和乙酸作为进水。在 pH 值为 8.2（±0.2）、温度为 26-28°C 和 SRT 为 4 天的条件下进行细菌培养，并采用微氧/好氧 MBBR 操作，在水力停留时间为 1 天、溶解氧（DO）范围为 0.2-3mg/L 和进水 C/N 和 C/P 比分别为 3.6 和 11 的条件下，可实现 100%的溶解性有机碳（DOC）、81-88%的总无机氮（TIN）和 83-86%的 P-PO 去除效率（RE）。乙酸的补充有利于微生物群落的多样化，而当在乙醇进料的 MBBR 中增加进料 C/N 比时，会观察到异养菌的过度生长。Illumina 测序显示，在 MBBR 生物膜和悬浮生物量中存在聚磷菌（PAO），如 Hydrogenophaga 和 Pseudomonas，而在研究过程中未鉴定出典型的亚硝酸盐菌（NOB）。
