Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710119, China.
State Key Laboratory of Physical Chemistry of Solid Surfaces, The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Collaborative Innovation Center of Chemistry for Energy Materials, Key Laboratory for Chemical Biology of Fujian Province, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China.
ACS Appl Mater Interfaces. 2021 Apr 7;13(13):15008-15016. doi: 10.1021/acsami.1c01568. Epub 2021 Mar 23.
Simultaneous detection of multi-biomarkers not only enhances the accuracy of disease diagnosis but also improves detection efficiency and reduces cost. It is vital to achieve portable, simple, low-cost, and simultaneous detection of biomarkers for point-of-care (POC) diagnostics in a low-resource setting. Herein, a multichannel paper chip-based gas pressure bioassay was developed for the simultaneous detection of multiple biomarkers by combining multichannel paper chips with a portable gas pressure meter. Four DNA tetrahedral probes (DTPs) were used as capture probes and were immobilized in different detection zones of the paper chips to improve hybridization efficiency and reduce nonspecific adsorption. The formation of a sandwich structure between target microRNAs (miRNAs), the capture probe, and platinum nanoparticles (PtNPs)-modified complementary DNA (PtNPs-cDNA) transformed biomolecular recognition into quantitative detection of gas pressure. Four lung cancer-related miRNAs were detected simultaneously by a portable gas pressure meter. There is a good linear relationship between gas pressure and the logarithm of miRNA concentration in the range of 10 pM to 100 nM. Compared with single-stranded DNA capture probe, the signal-to-noise (S/N) of DNA tetrahedral probes improved more than 3 times. Using ring-oven washing, the unbound reagents in all channels of the paper chip were simultaneously and continuously washed away, leading to a more cheap, simple, and fast separation than magnetic separation. Therefore, it offers a promising multichannel paper chip-based gas pressure bioassay for portable and simultaneous detection of multiple biomarkers.
同时检测多种生物标志物不仅可以提高疾病诊断的准确性，还可以提高检测效率并降低成本。在资源有限的环境中，实现生物标志物的便携、简单、低成本和同时检测对于即时检测（POC）诊断至关重要。在此，我们开发了一种基于多通道纸芯片的气压生物测定法，通过将多通道纸芯片与便携式气压计相结合，实现了多种生物标志物的同时检测。四个 DNA 四面体型探针（DTPs）被用作捕获探针，并固定在纸芯片的不同检测区域，以提高杂交效率并减少非特异性吸附。目标 microRNAs（miRNAs）、捕获探针和铂纳米粒子（PtNPs）修饰的互补 DNA（PtNPs-cDNA）之间三明治结构的形成将生物分子识别转化为气压的定量检测。通过便携式气压计同时检测了四种肺癌相关 miRNA。在 10 pM 到 100 nM 的范围内，气压与 miRNA 浓度的对数之间存在良好的线性关系。与单链 DNA 捕获探针相比，DNA 四面体型探针的信噪比（S/N）提高了 3 倍以上。通过环炉洗涤，纸芯片所有通道中的未结合试剂被同时连续地冲洗掉，与磁分离相比，这种方法更简单、更快速、更廉价。因此，它为便携式和同时检测多种生物标志物提供了一种有前途的基于多通道纸芯片的气压生物测定法。
