Institute of Forest Tree Genetic Breeding, Forestry College, Inner Mongolia Agricultural University, Xinjian Street 275, Hohhot City, Inner Mongolia, China.
Institute of Forestry, Academy of Agricultural and Forestry Sciences, Qinghai University, Ning da Road 251, Xining City, Qinghai Province, China.
Sci Rep. 2021 Mar 23;11(1):6694. doi: 10.1038/s41598-021-83948-w.
Picea mongolica, a native species with excellent industrial wood quality and strong sand-fixing capacity, may be utilized in construction of urban green spaces in arid areas in China. However, now the sustainability of the ecosystems where this species grows is at serious risk due to a lack of natural regeneration. In this study, we developed an efficient regeneration system and comprehensively analyzed various factors affecting somatic embryogenesis (SE) using zygotic embryos as explants. We identified the optimal plant growth regulators (PGRs) performance and the best donor trees (k81) for the generation of somatic embryos (SEMs). Additionally, we confirmed that the positive developmental window of SEMs initiation was at the end of July to early August, which is when zygotic embryos was at the late embryogeny. In this time period, specific transcripts associated with the regulation of epigenetic modifications, plant hormone-related genes, and embryonic development-related transcription factors play important roles for early SEMs initiation. These results may provide a valuable resource for vegetative propagation of Picea mongolica. Our results may help to establish a reliable protocol for plantlet regeneration, which may facilitate urban greening applications and industrialization in arid areas.
云杉是一种具有优良工业木材质量和强大固沙能力的本地物种，可能被用于中国干旱地区城市绿地的建设。然而，由于缺乏自然再生，该物种生长的生态系统的可持续性正面临严重威胁。在这项研究中，我们开发了一种高效的再生系统，并使用合子胚作为外植体，综合分析了影响体细胞胚胎发生（SE）的各种因素。我们确定了最佳的植物生长调节剂（PGRs）表现和最佳的供体树（k81），以产生体细胞胚胎（SEMs）。此外，我们还证实了 SEMs 起始的积极发育窗口在 7 月底至 8 月初，此时合子胚处于晚期胚胎发生阶段。在这个时间段内，与表观遗传修饰调控、植物激素相关基因和胚胎发育相关转录因子相关的特定转录本在早期 SEMs 起始中发挥重要作用。这些结果可能为云杉的营养繁殖提供有价值的资源。我们的研究结果可能有助于建立一个可靠的植物再生方案，这可能有助于干旱地区的城市绿化应用和产业化。
