Jilin Provincial Academy of Forestry Sciences, 3528 Linhe St., Changchun, 130033, Jilin, China.
College of Horticulture of Jilin Agricultural University, 2888 Xincheng St., Changchun, 130118, Jilin, China.
Sci Rep. 2023 Aug 14;13(1):13194. doi: 10.1038/s41598-023-39389-8.
Embryogenic tissue (ET) is important for genetic modification and plant re-generation. The proliferation ability and vigor of ET are crucial for plant propagation via somatic embryogenesis. In this study, ET was induced from mature zygotic embryos in blue spruce (Picea pungens Engelm.). There were significant differences in ET induction between two provenances, i.e. 78.8 ± 12.5% and 62.50 ± 12.8% respectively. Effects of 2,4-Dichlorophenoxy acetic acid (2,4-D), 6-Benzyl amino-purine (6-BA) and/or sucrose on ET proliferation and somatic embryo (SE) maturation were further investigated with four cell lines. The highest ET proliferation rate reached 1473.7 ± 556.0% biweekly. Concentrations of 2,4-D or 6-BA applied at tissue proliferation stage impacted SE maturation among the cell lines, whereas sucrose showed less effects. The highest rate, 408 ± 230 mature SEs/g FW, was achieved in SE maturation cultures. This research demonstrated that the culture conditions, i.e. the specific concentrations of 2,4-D and BA, at ET proliferation stage affected not only ET growth, but also the quality of ET for SE maturation. This study revealed the necessity and benefit in developing both the general and the genotype-specific protocols for efficient production of mature SEs, or somatic plants in blue spruce.
胚性组织（ET）对于遗传修饰和植物再生非常重要。ET 的增殖能力和活力对于通过体细胞胚胎发生进行植物繁殖至关重要。在这项研究中，从成熟的蓝云杉（Picea pungens Engelm.）合子胚中诱导出 ET。两个种源之间的 ET 诱导存在显著差异，分别为 78.8±12.5%和 62.50±12.8%。进一步用四个细胞系研究了 2,4-二氯苯氧基乙酸（2,4-D）、6-苄基氨基嘌呤（6-BA）和/或蔗糖对 ET 增殖和体细胞胚（SE）成熟的影响。每周两次，ET 的最高增殖率达到 1473.7±556.0%。在组织增殖阶段施加的 2,4-D 或 6-BA 的浓度会影响细胞系之间 SE 的成熟，而蔗糖的影响较小。在 SE 成熟培养物中达到最高速率，408±230 个成熟 SE/g FW。这项研究表明，培养条件，即 2,4-D 和 BA 的特定浓度，不仅影响 ET 的生长，而且影响 ET 用于 SE 成熟的质量。这项研究表明，在蓝云杉中，开发通用和基因型特异性的成熟 SE 或体细胞植物高效生产方案是必要的，也是有益的。
