Peng Xin, Wang Hantang, Yang Zhende
Guangxi Colleges and Universities Key Laboratory for Cultivation and Utilization of Subtropical Forest Plantation, Guangxi Key Laboratory of Forest Ecology and Conservation, College of Forestry, Guangxi University, Nanning 530004, China.
Insects. 2023 Sep 11;14(9):757. doi: 10.3390/insects14090757.
(1) Background: (Hymenoptera: Eulophidae) is a global invasive pest that seriously damages eucalyptus plants and has caused serious harm to forestry production in many countries. Two genotypically distinct lineages of have been detected outside of Australia, namely, lineage A and lineage B. However, the composition and abundance of endosymbiotic bacteria in are still unclear between lineages. Therefore, the purpose of this study was to compare the bacterial communities in female adults of of different lineages distributed in the same domain; (2) Methods: The PacBio Sequel II platform was used to compare bacterial community composition between lineages of by sequencing the V1-V9 region of the 16S rRNA gene, and fluorescence quantitative PCR was used to compare the relative expression of between lineages of ; (3) Results: A total of 437 operational taxonomic units (OTUs) were obtained. These OTUs were subdivided into 20 phyla, 32 classes, 77 orders, 129 families, and 217 genera. At the genus level, the dominant bacteria in lineage A and lineage B were and , respectively. There were differences in the bacterial community of between lineages, and the abundance and relative expression of in lineage A were significantly higher than those in lineage B; (4) Conclusions: There were differences in the bacterial community of between lineages, and the abundance and relative expression of in lineage A were significantly higher than those in lineage B.
(1) 背景：（膜翅目：姬小蜂科）是一种全球入侵性害虫，严重损害桉树植物，并在许多国家对林业生产造成了严重危害。在澳大利亚境外已检测到两种基因型不同的 谱系，即谱系A和谱系B。然而，不同谱系间 内共生细菌的组成和丰度仍不清楚。因此，本研究的目的是比较分布在同一区域的不同谱系 的雌性成虫中的细菌群落；(2) 方法：使用PacBio Sequel II平台，通过对16S rRNA基因的V1-V9区域进行测序来比较 不同谱系间的细菌群落组成，并使用荧光定量PCR来比较 不同谱系间 的相对表达；(3) 结果：共获得437个可操作分类单元（OTU）。这些OTU被细分为20个门、32个纲、77个目、129个科和217个属。在属水平上，谱系A和谱系B中的优势细菌分别为 和 。不同谱系间 的细菌群落存在差异，且谱系A中 的丰度和相对表达显著高于谱系B；(4) 结论：不同谱系间 的细菌群落存在差异，且谱系A中 的丰度和相对表达显著高于谱系B。
