Laboratory for Advanced Genomics, Ruđer Bošković Institute, Zagreb, Croatia.
Teaching Institute for Public Health, Rijeka, Croatia.
Arch Virol. 2021 Jun;166(6):1735-1739. doi: 10.1007/s00705-021-05029-7. Epub 2021 Mar 24.
We developed a next-generation SARS-CoV-2 sequencing platform and obtained the first SARS-CoV-2 sequences from patients in Croatia at the beginning of the COVID-19 outbreak in the spring of 2020. Integrating the sequencing and the epidemiological data, we show that patients were infected with different SARS-CoV-2 variants belonging to different clades (mostly G and GH). This result confirms that there was widespread virus transmission early in 2020. Interestingly, we identified a unique mutation resulting in a V13I substitution in Nsp5A, the main viral protease, in a patient who had not received antiviral therapy.
我们开发了一种下一代 SARS-CoV-2 测序平台，并在 2020 年春季 COVID-19 爆发初期从克罗地亚的患者中获得了第一批 SARS-CoV-2 序列。通过整合测序和流行病学数据，我们表明患者感染了属于不同进化枝（主要是 G 和 GH）的不同 SARS-CoV-2 变体。这一结果证实了 2020 年初病毒广泛传播的情况。有趣的是，我们在一名未接受抗病毒治疗的患者中发现了一种独特的突变，导致 Nsp5A（主要的病毒蛋白酶）中的 V13I 取代。
