College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
Key , Laboratory of Animal Diseases and Human Health of Sichuan Province, Chengdu, China.
Arch Virol. 2021 Jun;166(6):1769-1773. doi: 10.1007/s00705-021-04998-z. Epub 2021 Mar 24.
Porcine reproductive and respiratory syndrome (PRRS) is one of the most economically devastating viral diseases in the global pig industry. Recently, we isolated and plaque-purified porcine reproductive and respiratory syndrome virus (PRRSV) strain SC2020-1 from "aborted piglets" on a farm in Sichuan, China. To investigate the molecular biological characteristics of this strain, it was subjected to genome sequencing and analysis. The full-length genome sequence of strain SC2020-1 was 87.7% identical to that of the Lelystad strain (PRRSV type I protoype strain) and 82.2-84.8% identical to PRRSV type I isolates from China. NSP2, ORF3, and ORF4 were the most variable regions and contained discontinuous deletions or insertions when compared to other PRRSV type I strains. Phylogenetic analysis of the complete genome sequence showed that SC2020-1 clustered with PRRSV type I but outside of the three previously described branches (Lelystad virus-like, Amervac PRRS-like, and BJEU06-1-like). The Nsp2 gene was in the same branch with EUGDHD strains from China. This is the first report of PRRSV type I infection associated with abortion in sows in southwest China. Close attention should be paid to the prevention and control of this evolving virus.
猪繁殖与呼吸综合征（PRRS）是全球养猪业中最具经济破坏性的病毒性疾病之一。最近，我们从中国四川的一个农场的“流产仔猪”中分离并纯化了猪繁殖与呼吸综合征病毒（PRRSV）株 SC2020-1。为了研究该毒株的分子生物学特征，我们对其进行了基因组测序和分析。SC2020-1 株的全长基因组序列与 Lelystad 株（PRRSV 型 I 原型株）的同源性为 87.7%，与中国 PRRSV 型 I 分离株的同源性为 82.2-84.8%。NSP2、ORF3 和 ORF4 是最具变异性的区域，与其他 PRRSV 型 I 株相比，含有不连续的缺失或插入。全基因组序列的系统进化分析表明，SC2020-1 与 PRRSV 型 I 聚类，但不在先前描述的三个分支（Lelystad 病毒样、Amervac PRRS 样和 BJEU06-1 样）之外。Nsp2 基因与中国的 EUGDHD 株在同一分支。这是首次在中国西南地区报告与母猪流产相关的 PRRSV 型 I 感染。应密切关注这种不断进化的病毒的预防和控制。
