National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, China.
National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Engineering Research Center of Beijing, Beijing University of Technology, Beijing 100124, China.
Bioresour Technol. 2021 Jun;330:124936. doi: 10.1016/j.biortech.2021.124936. Epub 2021 Mar 6.
Partial-denitrification (PD; NO→NO) has recently been proposed to be a feasible choice of NO-N supply for anammox bacteria. In this study, an aerobic/anoxic process for treating domestic wastewater was operated for 176 days to evaluate the feasibility of using sludge fermentation liquid for partial denitrification of the wastewater. Results show that, with the ratio of C/N (COD/ NO-N) increased at anoxic stage, the average NO-N concentration in the effluent and nitrate-to-nitrite transformation ratio (NTR) at anoxic stage showed relative growth. High-throughput sequencing analysis demonstrated that the enhancement of PD can be explained by the increases of Thauera, Paracoccus and Enterobacteriaceae. Moreover, Candidatus_Brocadia (0.13%) was detected as the predominant anammox bacteria. Ex-situ isotopic tracing technique analysis assessed that the ratio of anammox role (ra%) was 7.29%. This study has a great potential for being coupled with the anammox bacteria for advanced nitrogen removal.
部分反硝化（PD；NO→NO）最近被提议为厌氧氨氧化菌提供 NO-N 供应的可行选择。本研究采用好氧/缺氧工艺处理生活污水 176 天，以评估利用污泥发酵液进行废水部分反硝化的可行性。结果表明，随着缺氧阶段 C/N 比（COD/NO-N）的增加，出水 NO-N 浓度和缺氧阶段硝酸盐到亚硝酸盐的转化率（NTR）呈相对增长。高通量测序分析表明，PD 的增强可以通过 Thauera、Paracoccus 和肠杆菌科的增加来解释。此外，检测到优势厌氧氨氧化菌为 Candidatus_Brocadia（0.13%）。体外同位素示踪技术分析评估，厌氧氨氧化作用的比例（ra%）为 7.29%。这项研究对于与厌氧氨氧化菌结合进行高级脱氮具有很大的潜力。
