New data on the mitochondrial genome of the winter crane fly (Diptera: Trichoceridae) with phylogenetic analysis.

The family Trichoceridae is a small group in Nematocera (Diptera) with 157 known species in the world. In this study, we report a complete mitochondrial genome sequence of a Trichoceridae species, which is a circular molecule of 16,094 bp with 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), two ribosomal RNA genes (rRNAs), and a 1063 bp long non-coding region (control region). Eight gene overlaps ranging from 1 to 8 bp in length and 16 small non-coding intergenic spacers ranging from 1 to 77 bp in length are found in the mitochondrial genome. The canonical start codons (ATN) for invertebrate mitochondrial genomes are found in 11 PCGs, except for which uses TTG and uses GTG. Stop codons of 12 PCGs are invariably complete TAA and TAG, while ends with a single thymine stop codon. Twenty-two tRNAs ranges from 64 bp to 72 bp, and the rRNAs are determined to be 1326 bp in length for and 788 bp in length for . Phylogenetic analysis reveals that the family Trichoceridae is a sister-group to the remaining Tipulomorpha, and the relationship between the other families is Pediciidae + (Limoniidae + (Cylindrotomidae + Tipulidae)).