Zhao Chuande, Kang Zehui, Xu Yuanyuan, Zhang Xiao
Key Lab of Integrated Crop Pest Management of Shandong Province, College of Plant Health and Medicine, Qingdao Agricultural University, Qingdao, China.
Mitochondrial DNA B Resour. 2021 Jan 13;6(1):115-118. doi: 10.1080/23802359.2020.1848478.
The genus Latreille, 1804 is recorded from Shandong Province, China for the first time with Yang Yang, 1988 found in Mount Kunyu, Shandong. In this study, we report the complete mitochondrial genome sequence of , representing the first mitochondrial genome of the subfamily Ctenophorinae (Diptera: Tipulidae), which is a circular molecule of 15,888 bp with an AT content of 77.6%. The mitochondrial genome contains 13 protein-coding genes (PCGs), 22 transfer RNA genes (tRNAs), two ribosomal RNA genes (rRNAs), and a non-coding region. Gene overlaps are found at nine gene junctions, ranging from 1 to 8 bp in length. The canonical mitochondrial start codons for invertebrate mitochondrial genomes are found in 12 PCGs, except for which uses the uncanonical start codons TCG. Stop codons of 10 PCGs are invariably complete TAA and TAG, while , and end with a single thymine stop codon. Phylogenetic analysis reveals that the Pediciidae is a sister group to the remaining Tipuloidea, the Cylindrotomidae has a sister-group relationship with the Tipulidae, and the Limoniidae is not a monophyletic clade.
1804年的拉特瑞尔属首次在中国山东省被记录，1988年杨扬在山东昆嵛山发现该属物种。在本研究中，我们报道了[物种名称]的完整线粒体基因组序列，这是大蚊亚科（双翅目：大蚊科）的首个线粒体基因组，它是一个15,888 bp的环状分子，AT含量为77.6%。线粒体基因组包含13个蛋白质编码基因（PCGs）、22个转运RNA基因（tRNAs）、两个核糖体RNA基因（rRNAs）和一个非编码区。在9个基因连接处发现了基因重叠，长度从1到8 bp不等。在12个PCGs中发现了无脊椎动物线粒体基因组的典型线粒体起始密码子，只有[特定基因名称]使用非典型起始密码子TCG。10个PCGs的终止密码子总是完整的TAA和TAG，而[特定基因名称1]、[特定基因名称2]和[特定基因名称3]以单个胸腺嘧啶终止密码子结尾。系统发育分析表明，大蚊科是其余大蚊总科的姐妹群，柱大蚊科与大蚊科具有姐妹群关系，网大蚊科不是一个单系类群。
