Jiang Ming, Wang Junfeng, Chen Minghui, Zhang Huijuan
College of Life Sciences, Taizhou University, Taizhou, P. R. China.
Scientific Research Management Center, East China Medicinal Botanical Garden, Lishui, P. R. China.
Mitochondrial DNA B Resour. 2021 Mar 11;6(3):762-768. doi: 10.1080/23802359.2021.1881835.
is a rare and endangered plant species with extremely small populations. In our study, we sequenced the complete chloroplast (CP) genome of and described its structural organization, and performed comparative genomic analyses with other Rosaceae CP genomes. The plastome of was 159,902 bp in length with 36.6% GC content and contained a pair of inverted repeats of 26,367 bp which separated a large single-copy region of 87,933 bp and a small single-copy region of 19,235 bp. The CP genome included 130 genes, of which 85 were protein-coding genes, 37 were transfer RNAs, and eight were ribosomal RNAs. Two genes, and , which are located at the borders of IRB/SSC and IRB/LSC, were presumed to be pseudogenes. A total of 61 SSRs were detected, of which, 59 loci were mono-nucleotide repeats, and two were di-nucleotide repeats. The phylogenic analysis indicated that the 14 Rosaceae species were divided into three groups, among which grouped with , , , , , and , and it was found to be a sister clade to . Our newly sequenced CP genome of will provide essential data for further studies on population genetics and biodiversity.
是一种种群数量极少的珍稀濒危植物物种。在我们的研究中，我们对[植物名称]的完整叶绿体（CP）基因组进行了测序，描述了其结构组织，并与其他蔷薇科CP基因组进行了比较基因组分析。[植物名称]的质体基因组长度为159,902 bp，GC含量为36.6%，包含一对26,367 bp的反向重复序列，它们将一个87,933 bp的大单拷贝区域和一个19,235 bp的小单拷贝区域分隔开来。CP基因组包含130个基因，其中85个是蛋白质编码基因，37个是转运RNA，8个是核糖体RNA。位于IRB/SSC和IRB/LSC边界的两个基因[基因名称1]和[基因名称2]被推测为假基因。共检测到61个简单序列重复（SSR），其中59个位点是单核苷酸重复，2个是二核苷酸重复。系统发育分析表明，14个蔷薇科物种分为三组，其中[植物名称]与[其他植物名称1]、[其他植物名称2]、[其他植物名称3]、[其他植物名称4]、[其他植物名称5]和[其他植物名称6]聚在一起，并且发现它是[另一植物名称]的姐妹进化枝。我们新测序的[植物名称]CP基因组将为进一步的种群遗传学和生物多样性研究提供重要数据。
