Huang Yuying, Yang Zerui, Huang Song, An Wenli, Li Jing, Zheng Xiasheng
DNA Barcoding Laboratory for TCM Authentication, Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
Traditional Chinese Medicine Gynecology Laboratory in Lingnan Medical Research Center, Guangzhou University of Chinese Medicine, Guangzhou 510410, China.
Plants (Basel). 2019 Apr 4;8(4):89. doi: 10.3390/plants8040089.
In the last decade, several studies have relied on a small number of plastid genomes to deduce deep phylogenetic relationships in the species-rich Myrtaceae. Nevertheless, the plastome of , an important representative plant of the Rhodomyrtus (DC.) genera, has not yet been reported yet. Here, we sequenced and analyzed the complete chloroplast (CP) genome of , which is a 156,129-bp-long circular molecule with 37.1% GC content. This CP genome displays a typical quadripartite structure with two inverted repeats (IRa and IRb), of 25,824 bp each, that are separated by a small single copy region (SSC, 18,183 bp) and one large single copy region (LSC, 86,298 bp). The CP genome encodes 129 genes, including 84 protein-coding genes, 37 tRNA genes, eight rRNA genes and three pseudogenes (, , ). A considerable number of protein-coding genes have a universal ATG start codon, except for and . Premature termination codons (PTCs) were found in one protein-coding gene, namely , which is rarely reported in the CP genome of plants. Phylogenetic analysis revealed that has a sister relationship with and . In conclusion, this study identified unique characteristics of the CP genome providing valuable information for further investigations on species identification and the phylogenetic evolution between and related species.
在过去十年中，多项研究依靠少数质体基因组来推断物种丰富的桃金娘科的深层系统发育关系。然而，桃金娘属重要代表性植物的质体基因组尚未见报道。在此，我们对该植物的完整叶绿体（CP）基因组进行了测序和分析，其为一个长度为156,129 bp的环状分子，GC含量为37.1%。该CP基因组呈现典型的四分体结构，有两个反向重复序列（IRa和IRb），各为25,824 bp，被一个小单拷贝区域（SSC，18,183 bp）和一个大单拷贝区域（LSC，86,298 bp）隔开。CP基因组编码129个基因，包括84个蛋白质编码基因、37个tRNA基因、8个rRNA基因和3个假基因（、、）。除了和外，相当数量的蛋白质编码基因具有通用的ATG起始密码子。在一个蛋白质编码基因（即）中发现了提前终止密码子（PTC），这在植物的CP基因组中鲜有报道。系统发育分析表明，与和具有姐妹关系。总之，本研究确定了该植物CP基因组的独特特征，为进一步开展物种鉴定以及该植物与相关物种间的系统发育进化研究提供了有价值的信息。
