肿瘤坏死因子-α rs1800629（-308G>A）基因多态性与肾病综合征之间无关联。

Lack of Association Between TNF-alpha rs1800629 (-308G > A) Polymorphism and Nephrotic Syndrome.

作者信息

Sadeghi-Bojd Simin, Hashemi Mohammad, Firoozi-Jahanigh Mahrokh, Rezaei Maryam, Sarani Hosna, Taheri Mohsen

机构信息

Genetics of Noncommunicable Disease Research Center, Zahedan University of Medical sciences, Zahedan, Iran.

出版信息

Iran J Kidney Dis. 2021 Mar;15(2):95-100.

PMID:33764319

Abstract

INTRODUCTION

Idiopathic Nephrotic Syndrome is a multifactorial disease that accompanying with immune system dysfunction. Cytokines as potent immunomodulators have a key role in pathogenesis of the disease. We aimed to evaluate the association between TNFα -308G > A polymorphism with Idiopathic Nephrotic Syndrome and its effect on the response to steroid therapy.

METHODS

This case-control study was performed on 168 patients with Nephrotic Syndrome and 153 healthy children. Genotyping of TNF-α rs1800629 (-308G > A) variant was detected by polymerase chain reaction restriction fragment length polymorphism method (PCR-RFLP).

RESULTS

The results revealed that there was no significant difference in allele (P > .05, OR = 0.92, 95% CI: 0.59 to 1.43) or genotype (P > .05, OR = 1.00; 95% CI: 0.62 to 1.65) frequency of TNF-α rs1800629 (-308G > A) between childhood cases of nephrotic syndrome and healthy controls. Also no association was found between genotype (P > .05, OR = 2.28; 95% CI: 1.03 to 5.04), and allele frequency (P > .05, OR = 1.93; 95% CI: 0.97 to 3.87) among the SSNS and SRNS groups.

CONCLUSION

Our results did not support any association between the TNF polymorphism and the risk of nephrotic syndrome in a sample of southeast Iranian population.

摘要

引言

特发性肾病综合征是一种伴有免疫系统功能障碍的多因素疾病。细胞因子作为强效免疫调节剂在该疾病的发病机制中起关键作用。我们旨在评估肿瘤坏死因子α（TNFα）-308G>A多态性与特发性肾病综合征之间的关联及其对类固醇治疗反应的影响。

方法

本病例对照研究对168例肾病综合征患者和153名健康儿童进行。采用聚合酶链反应-限制性片段长度多态性方法（PCR-RFLP）检测TNF-α rs1800629（-308G>A）变体的基因分型。

结果

结果显示，在儿童肾病综合征病例与健康对照之间，TNF-α rs1800629（-308G>A）的等位基因频率（P>.05，OR=0.92，95%CI：0.59至1.43）或基因型频率（P>.05，OR=1.00；95%CI：0.62至1.65）无显著差异。在单纯性肾病综合征（SSNS）和激素耐药型肾病综合征（SRNS）组之间，基因型频率（P>.05，OR=2.28；95%CI：1.03至5.04）和等位基因频率（P>.05，OR=1.93；95%CI：0.97至3.87）也未发现关联。