Venkatachalapathy Yogalakshmi, Suresh Praveenkumar Kochuthakidiyel, Balraj Thendral Hepsibha, Venkatesan Vettriselvi, Geminiganesan Sangeetha, Bhagam Indira, Priya C D Mohana
Department of Human Genetics, Sri Ramachandra Institute of Higher Education and Research, Chennai, India.
Department of Biochemistry, Ethiraj College for Women, Chennai, India.
Sci Rep. 2025 Aug 14;15(1):29797. doi: 10.1038/s41598-025-15387-w.
This study aims to explore the risk associated with the Tumour Necrosis Factor-Alpha (TNFα)-308 G/A (rs1800629) gene polymorphism in relation to Childhood Nephrotic Syndrome (NS). The primary goal of the meta-analysis was to investigate the relationship between Nephrotic Syndrome and the TNFα 308 G/A [rs1800629] polymorphism of the cytokine gene. We conducted a systematic search across electronic databases like PubMed and Google Scholar to collect data from five distinct case-control studies focused on the TNFα 308 G/A gene variant, covering the period from 2010 to 2022. By aggregating allele and genotype frequencies from these studies, we computed the 95% confidence interval of odds ratio [OR] to assess the strength of the association. To evaluate heterogeneity and potential publication bias in the selected studies, Stats Direct software was employed. The sample size encompassed 1,560 individuals, including 628 cases and 932 controls from five separate case-control studies. The TNFα allele-A displayed significant heterogeneity [I²=80%, 95% CI] when compared to the G allele, and a statistically significant pooled OR of 2.32 [P = 0.0056*] was observed. In the overall analysis, no significance association was found in the dominant model [P = 0.068], but a significant association was detected in the recessive model [P = 0.0096*]. Consistent findings were observed in the co-dominant model, where both AA vs. GG [P = 0.0075*] and GA vs. GG [P = 0.0219*] showed significant associations. These results suggest a potential increase in the risk of the disease associated with the polymorphism of TNFα 308 G/A. The small number of studies, high heterogeneity (I² = 80%), and limited ethnic diversity may affect the robustness and generalizability of findings. Additionally, language bias and lack of confounder adjustment limit interpretability. The TNFα 308 G/A polymorphism was identified as being associated with the risk of developing childhood NS.Registration: Registered in PROSPERO with ID: CRD420251083425.
本研究旨在探讨肿瘤坏死因子-α（TNFα）-308 G/A（rs1800629）基因多态性与儿童肾病综合征（NS）相关的风险。荟萃分析的主要目的是研究肾病综合征与细胞因子基因TNFα 308 G/A [rs1800629]多态性之间的关系。我们在PubMed和谷歌学术等电子数据库中进行了系统检索，以收集2010年至2022年期间五项针对TNFα 308 G/A基因变异的不同病例对照研究的数据。通过汇总这些研究中的等位基因和基因型频率，我们计算了比值比[OR]的95%置信区间，以评估关联强度。为了评估所选研究中的异质性和潜在的发表偏倚，我们使用了Stats Direct软件。样本量包括1560名个体，来自五项独立病例对照研究，其中628例为病例组，932例为对照组。与G等位基因相比，TNFα等位基因-A显示出显著的异质性[I²=80%，95%CI]，观察到合并OR为2.32，具有统计学意义[P = 0.0056*]。在总体分析中，显性模型未发现显著关联[P = 0.0
