Fujian Key Laboratory of Oral Diseases, Stomatological Key Lab of Fujian College and University, School and Hospital of Stomatology, Fujian Provincial Engineering Research Center of Oral Biomaterial, Fujian Medical University, Fujian, 350002, China.
Department of Stomatology, Stomatological Hospital of Xiamen Medical College, Fujian, 361003, China.
Lasers Surg Med. 2021 Oct;53(8):1105-1112. doi: 10.1002/lsm.23394. Epub 2021 Mar 25.
This study aimed to evaluate the effect of optimized irrigation with photon-induced photoacoustic streaming (PIPS) activation of different irrigants (distilled water or ethylenediaminetetraacetic acid [EDTA]) on smear layer removal, dentin microhardness, attachment morphology, and survival of stem cells of the apical papilla (SCAP) in an organotypic root canal model.
STUDY DESIGN/MATERIALS AND METHODS: A total of 144 standardized root segments were randomly allocated into 6 groups for irrigation: (i) NaOCl group, (ii) NaOCl + EDTA group, (iii) NaOCl + PIPS (distilled water) group, (iv) NaOCl + PIPS (EDTA) group, (v) NaOCl + EDTA + PIPS (distilled water) group, and (vi) NaOCl + EDTA + PIPS (EDTA) group. Each group was divided into four subgroups for assessment: (i) dentin cleanliness; (ii) dentin microhardness; (iii) cell attachment morphology; and (iv) viable SCAP quantification.
Compared with the control groups, the NaOCl + EDTA + PIPS (EDTA) group showed higher efficiency in smear layer removal and in increasing SCAP viability with more stretched cellular morphology. There were no statistically significant differences in either smear layer removal effect, dentin microhardness, attachment morphology, or survival of SCAP among the other groups when optimized with PIPS (distilled water or EDTA) (P > 0.05).
Our findings indicated that irrigation optimized with PIPS activation of EDTA for 40 seconds was conducive to smear layer removal without additional dentin microhardness decrease. Additionally, this irrigation created more cell-friendly dentin conditioning than other approaches, which was beneficial for the attachment and survival of SCAP. Lasers Surg. Med. © 2021 Wiley Periodicals LLC.
本研究旨在评估优化冲洗联合光子诱导光声流（PIPS）激活不同冲洗液（蒸馏水或乙二胺四乙酸[EDTA]）对粪肠球菌感染根管内玷污层去除、牙本质微硬度、根尖乳头干细胞（SCAP）黏附形态和存活的影响。
研究设计/材料与方法：将 144 个标准化根管段随机分为 6 组进行冲洗：（i）次氯酸钠组，（ii）次氯酸钠+EDTA 组，（iii）次氯酸钠+PIPS（蒸馏水）组，（iv）次氯酸钠+PIPS（EDTA）组，（v）次氯酸钠+EDTA+PIPS（蒸馏水）组，（vi）次氯酸钠+EDTA+PIPS（EDTA）组。每组再分为 4 个亚组进行评估：（i）牙本质清洁度；（ii）牙本质微硬度；（iii）细胞黏附形态；（iv）活的 SCAP 定量。
与对照组相比，次氯酸钠+EDTA+PIPS（EDTA）组在去除玷污层和提高 SCAP 活力方面效果更好，细胞形态更伸展。当优化冲洗液为 PIPS（蒸馏水或 EDTA）时，其他各组在去除玷污层效果、牙本质微硬度、黏附形态或 SCAP 存活率方面均无统计学差异（P>0.05）。
本研究结果表明，优化冲洗联合 40 秒 EDTA 激活 PIPS 有利于去除玷污层，且不会导致牙本质微硬度进一步下降。此外，与其他方法相比，这种冲洗方法更有利于创造对细胞友好的牙本质预处理环境，从而有利于 SCAP 的黏附和存活。激光外科医学杂志。© 2021 威利父子公司。
