School of Applied Biological Science, Hiroshima University, Kagamiyama, Higashi-Hiroshima, Japan.
Graduate School of Integrated Sciences for Life, Hiroshima University, Kagamiyama, Higashi-Hiroshima, Japan.
Biosci Biotechnol Biochem. 2021 Apr 24;85(5):1114-1120. doi: 10.1093/bbb/zbab036.
TRIPTYCHON (TRY) is one of the R3-MYB transcription factors. Its extended C-terminal 19 amino-acid region (CTRY) is considered to affect the ability of root hair differentiation in Arabidopsis. Here, to further understand the function of CTRY, it, together with GFP, was artificially fused with TRY homologs, CPC and ETC1, which do not contain such extended regions and induce root hair differentiation. Arabidopsis transgenic plants carrying the fusion proteins, CPC-CTRY-GFP and ETC1-CTRY-GFP, induced root hair differentiation as observed in those carrying the original proteins without CTRY. The expression levels of the fusion proteins in the transgenic plants were essentially the same as those of the original proteins, although their subcellular localization to nuclei of root epidermal cells was slightly changed by CTRY. Therefore, CTRY does not affect the ability of CPC and ETC1 to induce root hair differentiation when artificially fused, and its function may be restricted in TRY.
三叶草（TRY）是 R3-MYB 转录因子之一。其扩展的 C 端 19 个氨基酸区域（CTRY）被认为影响拟南芥根毛分化的能力。在这里，为了进一步了解 CTRY 的功能，将其与 GFP 一起人工融合到 CPC 和 ETC1 的同源物中，CPC 和 ETC1 不包含这样的扩展区域，也能诱导根毛分化。携带融合蛋白 CPC-CTRY-GFP 和 ETC1-CTRY-GFP 的拟南芥转基因植物诱导根毛分化，就像携带没有 CTRY 的原始蛋白一样。尽管 CTRY 略微改变了融合蛋白在根表皮细胞核内的亚细胞定位，但转基因植物中融合蛋白的表达水平与原始蛋白基本相同。因此，当人工融合时，CTRY 并不影响 CPC 和 ETC1 诱导根毛分化的能力，其功能可能在 TRY 中受到限制。
