Tominaga-Wada Rumi, Kurata Tetsuya, Wada Takuji
a Graduate School of Biosphere Sciences , Hiroshima University , Higashi-Hiroshima , Japan.
b Graduate School of Life Sciences , Tohoku University , Sendai , Japan.
Biosci Biotechnol Biochem. 2017 Sep;81(9):1762-1767. doi: 10.1080/09168451.2017.1343120. Epub 2017 Jun 23.
The CAPRICE (CPC) encodes an R3-type MYB transcription factor, which promotes root-hair differentiation. Previously, we showed that the CPC protein moves from the non-hair cell to the neighboring cell and induces root-hair differentiation in Arabidopsis. In addition, we proposed two cell-to-cell movement signal sequences, S1 and S2, in CPC. However, an S1:2xGFP:S2 chimera protein did not move between root epidermal cells. Here, we show that the S1 and S2 sequences do not confer cell-to-cell movement or nuclear localization ability to a GFP protein. The ENHANCER OF TRY AND CPC1 (ETC1) gene encodes the CPC homolog R3 MYB; this protein does not possess cell-to-cell movement ability or the S1 sequence. To elucidate whether the S1 sequence can induce cell-to-cell movement ability in ETC1, CPCp:S1:ETC1:2xGFP was constructed and introduced into Arabidopsis. Our results indicate that the addition of the S1 sequence was not sufficient for ETC1 to acquire cell-to-cell movement ability.
CAPRICE（CPC）编码一种R3型MYB转录因子，该因子促进根毛分化。此前，我们发现CPC蛋白从非毛细胞移动到相邻细胞，并诱导拟南芥根毛分化。此外，我们在CPC中提出了两个细胞间移动信号序列S1和S2。然而，一个S1:2xGFP:S2嵌合蛋白并未在根表皮细胞间移动。在此，我们表明S1和S2序列并未赋予GFP蛋白细胞间移动或核定位能力。TRY AND CPC1增强子（ETC1）基因编码CPC同源R3 MYB；该蛋白不具备细胞间移动能力或S1序列。为阐明S1序列是否能诱导ETC1的细胞间移动能力，构建了CPCp:S1:ETC1:2xGFP并导入拟南芥。我们的结果表明，添加S1序列不足以使ETC1获得细胞间移动能力。
