Zhejiang Provincial Key Laboratory of Soil Contamination Bioremediation, School of Environment and Resources, Zhejiang Agriculture and Forestry University, Hangzhou, 311300, China.
Nanjing Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, China.
Bull Environ Contam Toxicol. 2021 Oct;107(4):770-777. doi: 10.1007/s00128-021-03200-z. Epub 2021 Mar 25.
Visual sorting is a method that is widely used in microplastic analysis, but it has limitation in the quantification of small-size microplastics. Accelerated solvent extraction (ASE) which frequently used in the analysis of organic contaminants in soils and sediments was used here for the microplastics quantification. A recovery experiment using different spiked polymeric microplastics separately indicates that ASE was useful in the extraction of low-density and low-melting point polymeric microplastics. High recoveries and low matrix effects were observed for the polyethylene, polypropylene and polystyrene microplastics. A further comparison between ASE and visual sorting was conducted for seven soils from agricultural land used for long-term mulching with plastic films. The results confirmed that ASE was capable of microplastics quantification for farmland soils and polyethylene film in the ASE extracts could be identified by Fourier transform infrared spectroscopy. Meanwhile, ASE conducted on small samples (3.0 g) gave results that showed close agreement with the visual sorting method.
目视分拣是一种广泛应用于微塑料分析的方法，但在小尺寸微塑料的定量分析方面存在局限性。本研究采用加速溶剂萃取（ASE）法，该方法常用于土壤和沉积物中有机污染物的分析，用于微塑料的定量分析。使用不同浓度的掺杂聚合物微塑料进行的回收实验表明，ASE 法可有效提取低密度和低熔点聚合物微塑料。对于聚乙烯、聚丙烯和聚苯乙烯微塑料，观察到了较高的回收率和较低的基质效应。进一步对长期使用塑料薄膜进行覆盖的农业用地的 7 种土壤进行了 ASE 与目视分拣的比较。结果证实 ASE 可用于农田土壤中的微塑料定量分析，且 ASE 提取物中的聚乙烯薄膜可通过傅里叶变换红外光谱法进行鉴定。同时，对 3.0 g 小样本进行的 ASE 分析结果与目视分拣法具有高度一致性。
