Department of Otorhinolaryngology-Head and Neck Surgery, Biomedical Research Institute, Seoul National University Hospital, 101 Daehak-ro, Jongno-gu Seoul, 03080, Republic of Korea.
School of Pharmacy, Theranostic Macromolecules Research Center, Sungkyunkwan University, 2066 Seobu-ro, Jangan-gu, Suwon, 16419, Republic of Korea.
Tissue Eng Regen Med. 2021 Apr;18(2):225-233. doi: 10.1007/s13770-021-00335-2. Epub 2021 Mar 25.
Long segmental tracheal repair is challenging in regenerative medicine due to low adhesion of stem cells to tracheal scaffolds. Optimal transplantation of stem cells for tracheal defects has not been established. We evaluated the role of hyaluronic acid (HA) coating of tracheal scaffolds in mesenchymal stem cell (MSC) adhesion and tracheal regeneration in a rabbit model.
A three-dimensionally printed tubular tracheal prosthesis was incubated with dopa-HA-fluorescein isothiocyanate in phosphate-buffered saline for 2 days. MSCs were incubated with an HA-coated scaffold, and their adhesion was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. HA coated scaffolds with or without MSC seeding were transplanted at the circumferential tracheal defect in rabbits, and survival, rigid bronchoscopy, radiologic findings, and histologic findings were compared between the two groups.
HA-coated scaffolds showed better MSC adhesion than non-coated scaffolds. The HA-coated scaffolds with MSC group showed a wider airway and greater mucosal regeneration compared to the HA-coated scaffolds without MSC group.
HA coating of scaffolds can promote MSC adhesion and tracheal regeneration.
由于干细胞对气管支架的黏附性低，长节段气管修复在再生医学中具有挑战性。尚未建立用于气管缺损的干细胞的最佳移植方法。我们在兔模型中评估了透明质酸（HA）涂层气管支架在间充质干细胞（MSC）黏附和气管再生中的作用。
用多巴-HA-异硫氰酸荧光素在磷酸盐缓冲液中孵育三维打印管状气管假体 2 天。将 MSC 与 HA 涂层支架孵育，并通过 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐测定法评估其黏附性。在兔的环状气管缺损处移植 HA 涂层支架（有或没有 MSC 接种），比较两组的存活率、硬性支气管镜检查、影像学发现和组织学发现。
HA 涂层支架的 MSC 黏附性优于非涂层支架。与无 MSC 的 HA 涂层支架组相比，有 MSC 的 HA 涂层支架组气道更宽，黏膜再生更好。
支架的 HA 涂层可以促进 MSC 的黏附和气管的再生。
