反转录定量 PCR 进行基因表达分析。

Gene Expression Analysis by Reverse Transcription Quantitative PCR.

机构信息

Department of Life Science, Faculty of Science, Institute of Technology Sligo, Ash Lane, Co. Sligo, Ireland.

Department of Applied Sciences, Dundalk Institute of Technology, Dundalk, Co. Louth, Ireland.

出版信息

Methods Mol Biol. 2021;2283:61-74. doi: 10.1007/978-1-0716-1302-3_8.

DOI:10.1007/978-1-0716-1302-3_8

PMID:33765310

Abstract

The reverse transcription quantitative polymerase chain reaction (RT-qPCR) is a rapid detection technology that allows the amplification and quantification of specific RNA transcripts. RT-qPCR has increasingly been adopted for the detection and quantification of H. pylori across a range of sample types and applications. In addition, it is widely used to monitor host gene expression in cells and tissues in response to H. pylori infection . Outlined here is a two-step protocol that can be employed to analyze gene expression in H. pylori or H. pylori-infected samples.

摘要

逆转录定量聚合酶链反应（RT-qPCR）是一种快速检测技术，可扩增和定量特定的 RNA 转录本。RT-qPCR 已越来越多地用于检测和定量各种样本类型和应用中的 H. pylori。此外，它还广泛用于监测宿主基因在细胞和组织中对 H. pylori 感染的反应。这里概述了一种两步法方案，可用于分析 H. pylori 或 H. pylori 感染样本中的基因表达。