ARC Centre of Excellence for Nanoscale BioPhotonics, Department of Molecular Sciences, Macquarie University, North Ryde, NSW 2109, Sydney, Australia.
Biomacromolecules. 2021 Apr 12;22(4):1752-1755. doi: 10.1021/acs.biomac.0c01791. Epub 2021 Mar 25.
Silk is a popular protein biomaterial that has been used for various purposes such as tissue scaffolding, textiles and hydrogels. Various methods for covalent conjugation of functional molecules such as small molecule sensors and enzymes have been developed to create functionalized silk biomaterials. Here, we report a method for silk functionalization by using -GalNAc-transferases and azide-modified UDP-GalNAc nucleotide sugar substrates to incorporate azide functional groups onto the silk fibroin protein for functionalization with cycloalkynes by click chemistry. Using ppGalNAc-T1 and T13 enzymes, we could transfer azide-modified GalNAc monosaccharides onto fibroin and as a proof of concept, conjugated a strain-alkyne-functionalized Cy5 fluorophore to produce a Cy5-conjugated fibroin hydrogel. This facile azido functionalization of the silk has the potential for attachment of any cycloalkyne moiety.
丝绸是一种受欢迎的蛋白质生物材料，已被用于各种用途，如组织支架、纺织品和水凝胶。已经开发出各种用于共价偶联功能分子的方法，如小分子传感器和酶，以创建功能化的丝绸生物材料。在这里，我们报告了一种通过使用β-半乳糖基转移酶和叠氮修饰的 UDP-GalNAc 核苷酸糖底物将叠氮基团结合到丝素蛋白上的方法，用于通过点击化学将环炔基功能化到丝素蛋白上。使用 ppGalNAc-T1 和 T13 酶，我们可以将叠氮修饰的 GalNAc 单糖转移到丝素上，并作为概念验证，将应变炔基功能化的 Cy5 荧光团连接到丝素上，生成 Cy5 共轭丝素水凝胶。这种丝素的简便叠氮官能化具有连接任何环炔部分的潜力。
