Guo Dingyi, Sun Jie, Tian Minggang, Lin Weiying
Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Materials Science and Engineering, University of Jinan, Jinan, Shandong 250022, PR China.
Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Materials Science and Engineering, University of Jinan, Jinan, Shandong 250022, PR China; Guangxi Key Laboratory of Electrochemical Energy Materials, Institute of Optical Materials and Chemical Biology, School of Chemistry and Chemical Engineering, Guangxi University, Nanning, Guangxi 530004, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2021 Jul 5;255:119686. doi: 10.1016/j.saa.2021.119686. Epub 2021 Mar 10.
In this work, a red-emissive RNA ligand bearing two positive charges were developed for the visualization of mitochondrial depolarization, via the subcellular localization of the ligand molecules. The ligand with quinolinium moiety and strong electronic donor displays red fluorescence peaked at 630 nm. Meanwhile, the probe is concentrated in mitochondria of live cells due to the high mitochondrial membrane potential, and re-localizes into nucleolus upon mitochondrial depolarization owing to the affinity to RNA. In this manner, the decrease of mitochondrial membrane potential could be real-timely and in-situ monitored with the red-emissive probe. Particularly, two cations were decorated on the probe, which enables the fast response to mitochondrial depolarization with elevated sensitivity. Cell damage induced by HO was also successfully observed with the probe. We expect that the probe can promote researches on mitochondrial membrane potential, cell apoptosis, and relative areas.
在这项工作中，通过配体分子的亚细胞定位，开发了一种带有两个正电荷的红色发射RNA配体，用于可视化线粒体去极化。带有喹啉鎓部分和强电子供体的配体显示出在630nm处达到峰值的红色荧光。同时，由于线粒体膜电位高，该探针集中在活细胞的线粒体中，并且由于对RNA的亲和力，在线粒体去极化时重新定位到核仁中。通过这种方式，可以用红色发射探针实时原位监测线粒体膜电位的降低。特别地，在探针上修饰了两个阳离子，这使得对线粒体去极化的快速响应具有更高的灵敏度。该探针还成功地观察到了由HO诱导的细胞损伤。我们期望该探针能够促进线粒体膜电位、细胞凋亡及相关领域的研究。
