Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Materials Science and Engineering, University of Jinan, Jinan, Shandong 250022, P.R. China.
Analyst. 2019 Mar 25;144(7):2387-2392. doi: 10.1039/c9an00076c.
Mitochondria play central roles in plenty of biological processes, such as apoptosis, signaling, and cell differentiation. Mitochondrial depolarization is a significant sign of deterioration of intracellular status. Although many fluorescent probes for visualizing mitochondria have been delivered, two-photon red-emitting mitochondrial probes capable of detecting mitochondrial depolarization remain rare. In this work, by linking a pyrene moiety to a quinoline salt, we have constructed two-photon red-emitting mitochondrial probes, MVQ and HVQ. Between them, HVQ with a longer side chain exhibits higher hydrophobic properties, and can image mitochondria with high-fidelity due to the aggregation caused quenching effect. In cooperation with MTDR, a commercialized mitochondrial probe, HVQ, could be used to image the depolarization of mitochondria induced by a protonophore and hydrogen peroxide. We believe that HVQ can serve as a powerful tool for the investigation of mitochondria and mitochondrial membrane potential in fundamental biological research.
线粒体在许多生物过程中发挥着核心作用，如细胞凋亡、信号转导和细胞分化。线粒体去极化是细胞内状态恶化的一个重要标志。尽管已经有许多用于可视化线粒体的荧光探针被开发出来，但能够检测线粒体去极化的双光子红光发射线粒体探针仍然很少。在这项工作中，我们通过将一个芘基部分连接到一个喹啉盐上，构建了双光子红光发射线粒体探针 MVQ 和 HVQ。其中，具有更长侧链的 HVQ 表现出更高的疏水性，并且由于聚集引起的猝灭效应，可以高保真地对线粒体进行成像。与商业化的线粒体探针 MTDR 合作，HVQ 可用于成像质子载体和过氧化氢诱导的线粒体去极化。我们相信 HVQ 可以作为研究线粒体和线粒体膜电位的有力工具，在基础生物学研究中发挥重要作用。
