Forensic Medical Unit (FMU), Western Sydney Local Health District (WSLHD), Blacktown Hospital, Marcel Crescent, Blacktown, 2141, Sydney, Australia.
NSW Forensic and Analytical Science Service (FASS), P.O. Box 162, Lidcombe, NSW, 2141, Sydney, Australia.
J Forensic Leg Med. 2021 Apr;79:102130. doi: 10.1016/j.jflm.2021.102130. Epub 2021 Feb 3.
When providing a forensic assessment for a victim of sexual assault, a smear is made from any swab that has been collected with the purpose of finding sperm. A smear is made to assist the laboratory identify the optimal swab for DNA testing, to aid in the selection of downstream DNA testing options, to evaluate morphology and quantity of sperm in order to provide time since intercourse assessments, and to contribute additional information to the crime scene case when trying to determine how an offender's DNA came to be at the site where it was located. In the majority of Australian states, the forensic examiner makes the smear at the time of the forensic assessment (forensic history, examination, collection of specimens, documentation of injury and prophylactic treatment for sexually transmitted infections and pregnancy). The smear could be made, however, at a later time by forensic laboratory staff. There are some jurisdictions (for example in Queensland, Australia) where this is the preferred process. This research paper compared smear preparation by the forensic examiner with that prepared, at a later time, by the laboratory scientist. 143 pairs of high vaginal smears were analysed over a two and a half year period. Smears made by the examiner contained, for the most part, higher numbers of sperm, more background material and more intact sperm than smears made at a later date by laboratory scientific staff.
在对性侵犯受害者进行法医评估时，会从收集的拭子中制作涂片，目的是寻找精子。制作涂片有助于实验室确定最适合 DNA 测试的拭子，有助于选择下游 DNA 测试选项，评估精子的形态和数量，以提供性交后时间的评估，并在试图确定犯罪现场时提供额外的信息，以确定犯罪者的 DNA 是如何出现在其所在位置的。在澳大利亚的大多数州，法医检查人员在法医评估时（法医病史、检查、样本采集、损伤记录和预防性治疗性传播感染和怀孕）制作涂片。然而，涂片也可以由法医实验室工作人员在稍后时间制作。在某些司法管辖区（例如澳大利亚昆士兰州），这是首选的处理过程。本研究论文比较了法医检查人员和实验室科学家在稍后时间准备的涂片制备。在两年半的时间里，分析了 143 对高阴道涂片。检查人员制作的涂片在大多数情况下包含更多数量的精子、更多的背景材料和更完整的精子，而实验室科学人员稍后制作的涂片则更少。
