Department of Clinical Pathology, University Hospital of Odense, Odense, Denmark.
Int J Legal Med. 2011 Nov;125(6):887-90. doi: 10.1007/s00414-011-0612-3. Epub 2011 Sep 2.
In the routine clinical examination of sexual assault victims, apart from documenting physical evidence of abuse, securing evidence, typically DNA from blood, semen, or saliva, is an important part of the process. Often the presence of semen is considered a most interesting piece of evidence. Not only does it often contain enough DNA for DNA profiling, but it also strongly indicates that an actual sexual act has taken place. The examination of smear slides obtained in sexual assault cases is a time-consuming task especially for the less trained and in cases where the smear only contains few sperm cells. In this work the goal was to develop a procedure to rapidly visualize human sperm cells in smear slides with the use of bright-field microscopy. Using SPERM HY-LITER™ by Independent Forensics, human sperm cells are visualized using a fluorescently labeled mouse antibody which significantly decreases the time used to screen smears. By further using an EnVision+ (DAKO) protocol, it was possible to create an immunohistochemical staining method to visualize sperm cells in bright-field microscopy with the same level of certainty as when using SPERM HY-LITER™.
在对性侵犯受害者进行常规临床检查时，除了记录虐待的身体证据外，获取证据（通常是血液、精液或唾液中的 DNA）也是该过程的重要组成部分。通常，精液的存在被认为是最有趣的证据之一。它不仅通常包含足够用于 DNA 分析的 DNA，而且还强烈表明实际发生了性行为。检查性侵犯案件中获得的抹片是一项耗时的任务，尤其是对于训练较少的人员，并且在抹片仅包含少量精子细胞的情况下。在这项工作中，目标是开发一种使用明场显微镜快速可视化抹片中人类精子细胞的程序。使用 Independent Forensics 的 Sperm HY-LITER™，使用荧光标记的小鼠抗体来可视化人类精子细胞，这大大减少了筛查涂片所需的时间。通过进一步使用 EnVision+（DAKO）方案，可以创建一种免疫组织化学染色方法，以便在明场显微镜下可视化精子细胞，其确定性与使用 Sperm HY-LITER™ 相同。
