Infectious Disease Unit , Specialty Internal Medicine, Johns Hopkins Aramco Healthcare, 31311 Dhahran, Saudi Arabia.
Infectious Disease Division, Department of Medicine, Indiana University School of Medicine, 46202 Indianapolis, IN, USA.
Trans R Soc Trop Med Hyg. 2022 Jan 19;116(1):50-53. doi: 10.1093/trstmh/trab055.
The molecular epidemiology of resistance of carbapenem-resistant Enterobacteriaceae (CRE) and Pseudomonas aeruginosa are important in the study of multidrug-resistant bacteria. We evaluate the prevalence of the different mechanisms of CRE in a hospital in Saudi Arabia.
Carbapenem non-susceptible isolates of Enterobacteriaceae and Pseudomonas aeruginosa were tested by real-time PCR for the detection of genes responsible for beta-lactam resistance.
There were a total of 200 isolates with carbapenem non-susceptibility and these were Klebsiella pneumoniae (n=96, 48%), Escherichia coli (n=51, 25.5%) and Pseudomonas aeruginosa (n=45, 22.5%). The detected carbapenemases were oxacillinase-48 (OXA-48) (n=83, 41.5%), New Delhi metallo-β-lactamase (NDM) (n=19, 2.5%) and both NDM and OXA-48 (n=5, 2.5%). The other carbapenemases were imipenemase (n=1, 0.5%), Verona integrin encoded metallo-β-lactamase (n=6, 3%) and Klebsiella pneumoniae carbapenemase (n=1, 0.5%), but none were detected in 86 isolates (43%).
The most common carbapenemases were OXA-48 and a significant percentage had no detectable genes. These data will help in the selection of new antimicrobial therapies.
耐碳青霉烯类肠杆菌科（CRE）和铜绿假单胞菌的耐药分子流行病学在研究多药耐药菌中非常重要。我们评估了沙特阿拉伯一家医院中不同 CRE 机制的流行情况。
采用实时 PCR 法检测耐碳青霉烯类肠杆菌科和铜绿假单胞菌的β-内酰胺耐药基因。
共有 200 株对碳青霉烯类药物不敏感的分离株，其中肺炎克雷伯菌（n=96，48%）、大肠埃希菌（n=51，25.5%）和铜绿假单胞菌（n=45，22.5%）。检测到的碳青霉烯酶包括 oxacillinase-48（OXA-48）（n=83，41.5%）、新德里金属β-内酰胺酶（NDM）（n=19，2.5%）以及 NDM 和 OXA-48（n=5，2.5%）。其他碳青霉烯酶包括亚胺培南酶（n=1，0.5%）、 Verona 整合子编码金属β-内酰胺酶（n=6，3%）和肺炎克雷伯菌碳青霉烯酶（n=1，0.5%），但在 86 株分离株中未检测到（43%）。
最常见的碳青霉烯酶是 OXA-48，有相当比例的分离株没有检测到基因。这些数据将有助于选择新的抗菌治疗方法。
