一种用于检测葡萄球菌分离株中毒性休克综合征毒素（TSST-1）的改良酶联免疫吸附测定法。

A modified ELISA for the detection of toxic shock syndrome toxin (TSST-1) in staphylococcal isolates.

作者信息

Fey H, Pfister H, Boerlin P

机构信息

Institut für Veterinär-Virologie der Universität, Bern, Switzerland.

出版信息

Zentralbl Bakteriol Mikrobiol Hyg A. 1988 Jan;267(3):363-6. doi: 10.1016/s0176-6724(88)80052-x.

DOI:10.1016/s0176-6724(88)80052-x

PMID:3376616

Abstract

A modified ELISA for the detection of S. aureus strains producing toxic shock syndrome toxin (TSST-1) is described. Polystyrene balls are coated with specific sheep antibody and incubated with an over-night culture of suspected colonies. Biotinylated second antibody and an Avidin/biotinylated enzyme system are used to obtain an easily readable qualitative reaction.

摘要

本文描述了一种改良的酶联免疫吸附测定法（ELISA），用于检测产生中毒性休克综合征毒素（TSST-1）的金黄色葡萄球菌菌株。用特异性绵羊抗体包被聚苯乙烯球，并与疑似菌落的过夜培养物一起孵育。使用生物素化二抗和抗生物素蛋白/生物素化酶系统来获得易于读取的定性反应。