Department of Technology, Savitribai Phule Pune University, Pune, 411007, India; Research and Development Department, Serum Institute of India Pvt. Ltd, Hadapsar, Pune, 411028, Maharashtra, India.
Division of Biochemistry, Department of Chemistry, Savitribai Phule Pune University, Pune, 411007, India.
Carbohydr Polym. 2021 Jun 1;261:117859. doi: 10.1016/j.carbpol.2021.117859. Epub 2021 Feb 25.
Development of an effective purification process in order to provide low cost and high-quality vaccine is the necessity of glycoconjugate vaccine manufacturing industries. In the present study, we have attempted to develop a method for simultaneous purification and depolymerization process for capsular polysaccharides (CPS) derived from Streptococcus pneumoniae serotype 2. Trifluoroacetic acid (TFA) was used to precipitate impurities which were then removed by centrifugation. It was observed that the TFA treatment could simultaneously depolymerize the CPS and purify it. The purified and depolymerized CPS was analyzed for its purity, structural identity and conformity, molecular size, antigenicity to meet desired quality specifications. The obtained results showed that the purification and depolymerization of S. pneumoniae serotype 2 CPS did not affect the antigenicity of CPS.
为了提供低成本、高质量的疫苗，开发有效的纯化工艺是糖缀合物疫苗制造行业的必然需求。在本研究中，我们尝试开发一种同时用于纯化和降解来自肺炎链球菌血清型 2 的荚膜多糖 (CPS) 的方法。三氟乙酸 (TFA) 用于沉淀杂质，然后通过离心去除。结果表明，TFA 处理可以同时降解 CPS 并对其进行纯化。对纯化和降解的 CPS 进行纯度、结构同一性和一致性、分子大小、抗原性分析，以满足所需的质量规格。所得结果表明，肺炎链球菌血清型 2 CPS 的纯化和降解不影响 CPS 的抗原性。
