Department of Joint and Orthopedics, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong, PR China; Department of Biomedical Engineering, Southern University of Science and Technology, Shenzhen, Guangdong, PR China; Shenzhen Key Laboratory of Cell Microenvironment, PR China.
Shenzhen Key Laboratory of Cell Microenvironment, PR China; Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, PR China.
Carbohydr Polym. 2021 Jun 1;261:117869. doi: 10.1016/j.carbpol.2021.117869. Epub 2021 Feb 26.
Osteoarthritis (OA) is an age-related joint disorder and one of the leading causes of physical disability. In this study, we designed and synthesized a new polysaccharide complex, carboxymethyl chitosan strontium (CMCS-Sr), which is believed to have positive effects on relieving OA. The synthesized CMCS-Sr was structurally verified by SEM, EDS, FTIR, etc. The therapeutic effects of CMCS-Sr were evaluated using various biological experiments. The cell viability and apoptosis results reveal that CMCS-Sr can significantly promote the proliferation and suppress OA chondrocytes apoptosis in vitro. The immunofluorescence staining results suggest that CMCS-Sr facilitates the promotion of the secretion of Type II collagen (Col-II). The transcriptomic results support the observed positive effects of CMCS-Sr on inhibiting chondrocytes apoptosis and alleviating inflammatory reactions. Moreover, animal study demonstrates that CMCS-Sr effectively reduced articular cartilage damage and subchondral bone degradation. Therefore, we propose the use of CMCS-Sr as a promising candidate for relieving OA.
骨关节炎（OA）是一种与年龄相关的关节疾病，也是导致身体残疾的主要原因之一。在这项研究中，我们设计并合成了一种新型多糖复合物，即羧甲基壳聚糖锶（CMCS-Sr），据信它对缓解 OA 有积极作用。通过 SEM、EDS、FTIR 等方法对合成的 CMCS-Sr 进行了结构验证。通过各种生物学实验评估了 CMCS-Sr 的治疗效果。细胞活力和细胞凋亡结果表明，CMCS-Sr 可以显著促进 OA 软骨细胞的体外增殖并抑制其凋亡。免疫荧光染色结果表明，CMCS-Sr 促进 II 型胶原（Col-II）的分泌。转录组学结果支持 CMCS-Sr 抑制软骨细胞凋亡和减轻炎症反应的观察到的积极作用。此外，动物研究表明 CMCS-Sr 可有效减轻关节软骨损伤和软骨下骨降解。因此，我们提出将 CMCS-Sr 用作缓解 OA 的有前途的候选药物。
