Nano-Biopolymers Research Laboratory, School of Chemical Engineering, College of Engineering, University of Tehran, P. O. Box: 11155-4563, Tehran, Iran.
Nano-Biopolymers Research Laboratory, School of Chemical Engineering, College of Engineering, University of Tehran, P. O. Box: 11155-4563, Tehran, Iran.
Carbohydr Polym. 2021 Jun 1;261:117889. doi: 10.1016/j.carbpol.2021.117889. Epub 2021 Mar 3.
This work was aimed to synthesize novel crosslinked carboxymethyl chitosan nanoparticles (CMCS NPs) containing metformin hydrochloride (MET) using microfluidics (MF) and evaluate their performance for diabetes therapy. The field emission-scanning electron microscopy (FE-SEM) images and dynamic light scattering (DLS) results showed that the NPs average size was 77 ± 19 nm with a narrow size distribution. They exhibited a high encapsulation efficiency (∼90 %) and the controlled drug release while crosslinking using CaCl. Eventually, the in vivo assessments dedicated an increased body weight up to 7.94 % and a decreased blood glucose level amount of 43.58 % for MF MET-loaded CMCS NPs with respect to the free drug in diabetic rats. Also, the results of histopathological studies revealed the size of the pancreatic islets to be 2.32 μm and β cells intensity to be 64 cells per islet for the diabetic rats after treating with the MF-based sample. These data were close to those obtained for the healthy rats.
这项工作旨在使用微流控技术（MF）合成含有盐酸二甲双胍（MET）的新型交联羧甲基壳聚糖纳米粒子（CMCS NPs），并评估其在糖尿病治疗中的性能。场发射扫描电子显微镜（FE-SEM）图像和动态光散射（DLS）结果表明，纳米粒子的平均粒径为 77 ± 19nm，具有较窄的粒径分布。它们表现出高包封效率（约 90%）和控制药物释放，同时使用 CaCl2 交联。最终，体内评估显示，与游离药物相比，糖尿病大鼠中载有 MF-MET 的 CMCS NPs 可使体重增加高达 7.94%，血糖水平降低 43.58%。此外，组织病理学研究结果表明，用基于 MF 的样品治疗糖尿病大鼠后，胰岛的大小为 2.32μm，每个胰岛的β细胞强度为 64 个细胞。这些数据接近健康大鼠的结果。
