Unit of Human Bacterial Diseases, Sciensano, Belgium.
Unit of Human Bacterial Diseases, Sciensano, Belgium.
J Microbiol Methods. 2021 May;184:106190. doi: 10.1016/j.mimet.2021.106190. Epub 2021 Mar 22.
Rapid differentiation of the AviPro Salmonella VAC T strain from wild-type Salmonella ser. Typhimurium isolates is essential for the monitoring of veterinary isolates and targeted control actions. The distinction between the two strain types is routinely made by phenotypic antimicrobial resistance testing, but this sometime leads to ambiguous results with major economic implications. In this study, we used whole-genome sequencing to identify conserved and specific mutations in resistance and virulence genes which enable to distinguish field and vaccine strains. Based on this information, we developed and validated (n = 199) a Luminex-based assay targeting seven specific single-nucleotide polymorphisms. This molecular test is able to distinguish both Salmonella ser. Typhimurium types with 100% sensitivity and specificity within one working day.
快速区分 AviPro 沙门氏菌 VAC T 株与野生型鼠伤寒沙门氏菌分离株对于兽医分离株的监测和靶向控制措施至关重要。两种菌株类型的区别通常通过表型抗菌药物耐药性测试来进行，但有时会导致具有重大经济影响的结果出现歧义。在这项研究中，我们使用全基因组测序来鉴定耐药性和毒力基因中的保守和特定突变，这些突变可用于区分田间菌株和疫苗菌株。基于这些信息，我们开发并验证了（n=199）一种基于 Luminex 的检测方法，该方法靶向七个特定的单核苷酸多态性。该分子检测方法在一个工作日内能够 100%灵敏且特异地区分血清型鼠伤寒沙门氏菌的两种类型。
