Ceyssens Pieter-Jan, Mueller-Doblies Doris, Mattheus Wesley
Unit of Human Bacterial Diseases, Sciensano, 1050 Brussels, Belgium.
Elanco Austria GmbH, Quartier Belvedere Central, 1100 Vienna, Austria.
Vet Sci. 2024 Mar 6;11(3):120. doi: 10.3390/vetsci11030120.
The timely differentiation of the AviPro Salmonella VAC T and VAC E strains from the wild-type ser. Typhimurium and ser. Enteritidis isolates is crucial for effectively monitoring veterinary isolates. Currently, the distinction between field and vaccine strains has been conducted routinely via phenotypic antimicrobial resistance testing since the vaccines were first introduced more than 20 years ago, and the differentiation based on the antimicrobial resistance profile is still a valid and well-established method. However, an alternative method was sought for those laboratories that prefer a PCR-based method for logistic and/or operational reasons. In this study, we developed two triplex Real-Time PCR reactions that targeted conserved and specific mutations and, therefore, enabled the reliable differentiation of field and vaccine strains. To validate the effectiveness of both assays, we extensively tested them on a dataset consisting of 405 bacterial strains. The results demonstrated a 100% sensitivity and specificity for distinguishing both ser. Typhimurium and Enteritidis, although a confirmed culture is required.
及时区分AviPro沙门氏菌VAC T和VAC E菌株与野生型鼠伤寒血清型和肠炎血清型分离株，对于有效监测兽医分离株至关重要。自20多年前首次引入疫苗以来，目前通过表型抗菌药物耐药性检测常规区分田间菌株和疫苗菌株，基于抗菌药物耐药谱的区分仍然是一种有效且成熟的方法。然而，对于那些因后勤和/或操作原因更喜欢基于PCR方法的实验室，人们寻求了一种替代方法。在本研究中，我们开发了两种针对保守和特定突变的三重实时PCR反应，从而能够可靠地区分田间菌株和疫苗菌株。为了验证这两种检测方法的有效性，我们在由405株细菌组成的数据集上对它们进行了广泛测试。结果表明，尽管需要经过确认的培养物，但区分鼠伤寒血清型和肠炎血清型的灵敏度和特异性均为100%。
