Applied Phycology and Biotechnology, CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI), Council of Scientific and Industrial Research (CSIR), Bhavnagar, Gujarat 364001, India.
Applied Phycology and Biotechnology, CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI), Council of Scientific and Industrial Research (CSIR), Bhavnagar, Gujarat 364001, India.
Gene. 2021 Jun 20;786:145597. doi: 10.1016/j.gene.2021.145597. Epub 2021 Mar 23.
We hereby report in planta function characterization of a novel galactosyl transferase-like (SbGalT) gene from Salicornia brachiata for enhanced abiotic stress tolerance. The SbGalT gene had an open reading frame of 1563 bp. The ectopic expression of SbGalT gene in tobacco improved the seed germination, seedling growth, biomass accumulation and potassium/sodium ratio under salt and osmotic stress. The SbGalT over-expression delayed stress-induced senescence, pigment break-down and ion induced cytotoxicity in tobacco. Higher contents of organic solutes and potassium under stress maintained the osmotic homeostasis and relative water content in tobacco. Higher activity of antioxidant enzymes under stress in transgenic tobacco curtailed the accumulation of reactive oxygen species (ROS) and maintained the membrane integrity. The chlorophyll a fluorescence transient indicated no effects of the imposed strengths of stress on basal state of photosystem (PS) I in transgenic tobacco over-expressing the SbGalT gene. Due to improved membrane integrity, the transgenic tobacco exhibited improved photosynthesis, stomatal conductance, intercellular CO, transpiration, maximum quantum yield and operating efficiency of PSII, electron transport, photochemical and non-photochemical quenching. In agreement with photosynthesis, physiological health, tolerance index and growth parameters, transgenic tobacco accumulated higher contents of sugar, starch, amino acid, polyphenol and proline under stress conditions. The multivariate data analysis exhibited significant statistical distinctions among osmotic adjustment, physiological health and growth, and photosynthetic responses in control and SbGalT transgenic tobacco under stress conditions. The results strongly indicated novel SbGalT gene as a potential candidate for developing the smart agriculture.
我们在此报告来自滨藜（Salicornia brachiata）的新型半乳糖基转移酶样（SbGalT）基因在提高非生物胁迫耐受性中的体内功能特征。SbGalT 基因的开放阅读框为 1563bp。SbGalT 基因在烟草中的异位表达提高了盐和渗透胁迫下种子的萌发、幼苗生长、生物量积累和钾/钠比。SbGalT 过表达延缓了烟草中胁迫诱导的衰老、色素分解和离子诱导的细胞毒性。胁迫下较高的有机溶质和钾含量维持了烟草的渗透平衡和相对含水量。胁迫下转基因烟草中抗氧化酶活性的提高抑制了活性氧（ROS）的积累，维持了膜的完整性。叶绿素 a 荧光瞬变表明，在过表达 SbGalT 基因的转基因烟草中，胁迫施加的强度对光系统（PS）I 的基础状态没有影响。由于膜完整性的提高，转基因烟草表现出改善的光合作用、气孔导度、胞间 CO、蒸腾作用、PSII 的最大量子产量和操作效率、电子传递、光化学和非光化学猝灭。与光合作用一致，在胁迫条件下，转基因烟草积累了更高含量的糖、淀粉、氨基酸、多酚和脯氨酸，从而提高了生理健康、胁迫指数和生长参数。多元数据分析表明，在胁迫条件下，控制和 SbGalT 转基因烟草的渗透调节、生理健康和生长以及光合响应之间存在显著的统计学差异。这些结果强烈表明新型 SbGalT 基因是开发智能农业的潜在候选基因。
