Division of Applied Phycology and Biotechnology, CSIR-Central Salt and Marine Chemicals Research Institute, G. B. Marg, Bhavnagar 364002, India.
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
Cells. 2021 Dec 27;11(1):62. doi: 10.3390/cells11010062.
is an extreme halophyte that commonly grows on marsh conditions and is also considered a promising resource for drought and salt-responsive genes. To unveil a glimpse of stress endurance by plants, it is of the utmost importance to develop an understanding of stress tolerance mechanisms. 'Early Responsive to Dehydration' (ERD) genes are defined as a group of genes involved in stress tolerance and the development of plants. To increase this understanding, parallel to this expedited thought, a novel gene was cloned from , characterized, and functionally validated in the model plant tobacco. The study showed that is a plasma-membrane bound protein, and its overexpression in tobacco plants improved salinity and osmotic stress tolerance. Transgenic plants showed high relative water, chlorophylls, sugars, starch, polyphenols, proline, free amino acids, and low electrolyte leakage and HO content compared to control plants (wild type and vector control) under different abiotic stress conditions. Furthermore, the transcript expression of antioxidant enzyme encoding genes , , , and showed higher expression in transgenic compared to wild-type and vector controls under varying stress conditions. Overall, the overexpression of a novel early responsive to dehydration stress protein 4-encoding gene () enhanced the tolerance of the plant against multiple abiotic stresses. In conclusion, the overexpression of the gene mitigates plant physiology by enduring stress tolerance and might be considered as a promising key gene for engineering salinity and drought stress tolerance in crops.
是一种极端的嗜盐植物，通常生长在沼泽条件下，也被认为是干旱和盐响应基因的有前途的资源。为了揭示植物的抗逆能力，了解植物的耐受力机制至关重要。“早期响应脱水”（ERD）基因被定义为一组参与植物耐受力和发育的基因。为了增加对此的理解，与这一快速思维并行，从 中克隆了一个新的 基因，在模式植物烟草中进行了鉴定和功能验证。研究表明， 是一种质膜结合蛋白，其在烟草植物中的过表达提高了盐度和渗透胁迫耐受性。与对照植物（野生型和载体对照）相比，转基因植物在不同非生物胁迫条件下表现出高相对水、叶绿素、糖、淀粉、多酚、脯氨酸、游离氨基酸和低电解质泄漏和 HO 含量。此外，在不同胁迫条件下，抗氧化酶编码基因 、 、 、 的转录表达在转基因植物中比野生型和载体对照中的表达更高。总的来说，新型早期响应脱水胁迫蛋白 4 编码基因（）的过表达增强了植物对多种非生物胁迫的耐受性。总之，过表达 基因通过耐受胁迫来缓解植物的生理机能，可能被认为是工程化作物耐盐和干旱胁迫的有前途的关键基因。
