Liu Bing, Cong Chenyang, Ma Yan, Ma Xiaohua, Zhang Han, Wang Jiawei
Department of Ophthalmology, the Second Hospital of Shandong University, Cheeloo College of Medicine, Shandong University, Jinan, China.
Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine, Jinan, China.
Eye (Lond). 2022 Mar;36(3):575-584. doi: 10.1038/s41433-021-01507-z. Epub 2021 Mar 25.
To investigate the differences in lncRNAs expression in whole blood between diabetic retinopathy (DR) patients and healthy subjects, and to evaluate the potential value of lncRNAs as a diagnostic biomarker for proliferative diabetic retinopathy (PDR).
A series of 34 PDR patients, 34 patients with non-proliferative DR (NPDR) and 34 healthy participants were enroled. Differentially expressed lncRNAs were demonstrated using high-throughput sequencing and validated using qRT-PCR. Gene Ontology (GO) was performed to explore the possible biological function of the differentially expressed lncRNAs. lncRNA/mRNA coexpression network was built to determine the targets of differentially expressed lncRNAs. Receiver operating characteristic (ROC) analysis was utilized to evaluate the diagnostic value of lncRNAs for PDR.
We identified 175 and 179 differentially expressed lncRNAs in PDR patients compared with control samples and NPDR patients, respectively. GO analysis showed that the various metabolic processes were possibly influenced by these dysregulated lncRNAs. Using the differently expressed lncRNAs data, we further identified 82 overlapping lncRNAs in PDR patients with NPDR and control subjects. Part of these overlapping lncRNAs was significantly correlated with nuclear factor kappa B (NF-κB) and Wnt signal pathways. ROC curves were constructed for two upregulated lncRNAs and the ROC analysis indicated that both of them had potential diagnostic value and could distinguish PDR from control subjects and NPDR patients.
LncRNAs expression was altered in PDR patients compared with NPDR and control subjects. Moreover, it provides a resource that lncRNAs might be novel diagnostic and prognostic biomarker for PDR.
研究糖尿病视网膜病变（DR）患者与健康受试者全血中长链非编码RNA（lncRNAs）表达的差异，并评估lncRNAs作为增殖性糖尿病视网膜病变（PDR）诊断生物标志物的潜在价值。
纳入34例PDR患者、34例非增殖性DR（NPDR）患者和34名健康参与者。使用高通量测序展示差异表达的lncRNAs，并通过qRT-PCR进行验证。进行基因本体论（GO）分析以探索差异表达lncRNAs可能的生物学功能。构建lncRNA/mRNA共表达网络以确定差异表达lncRNAs的靶标。利用受试者工作特征（ROC）分析评估lncRNAs对PDR的诊断价值。
与对照样本和NPDR患者相比，我们分别在PDR患者中鉴定出175个和179个差异表达的lncRNAs。GO分析表明，各种代谢过程可能受到这些失调lncRNAs的影响。利用差异表达的lncRNAs数据，我们在PDR患者与NPDR患者及对照受试者中进一步鉴定出82个重叠的lncRNAs。这些重叠lncRNAs中的一部分与核因子κB（NF-κB）和Wnt信号通路显著相关。为两个上调的lncRNAs构建了ROC曲线，ROC分析表明它们都具有潜在的诊断价值，并且可以区分PDR与对照受试者和NPDR患者。
与NPDR患者和对照受试者相比，PDR患者中lncRNAs的表达发生了改变。此外，这提供了一种资源，即lncRNAs可能是PDR新的诊断和预后生物标志物。
